Dermatofity to grzyby, które atakują i rozmnażają się w zrogowaciałej skórze ssaków, w tym ludzi, często powodując zaraźliwe infekcje. Gatunki o znaczeniu medycznym należą do rodzajów Microsporum, Trichophyton, Epidermophyton (w stanie anamorficznym) i Arthroderma (w stanie telomorficznym), które tradycyjnie identyfikowano na podstawie ich morfologii i cech biochemicznych. Niemniej jednak ograniczenia związane z różnicowaniem blisko spokrewnionych czynników na poziomie gatunków i szczepów zostały ostatnio przezwyciężone dzięki badaniom molekularnym.
Rzeczywiście, dokładna identyfikacja dermatofitów ma kluczowe znaczenie dla ustanowienia skutecznych programów kontroli i zapobiegania, a także dla określenia najbardziej odpowiednich i skutecznych terapii przeciwgrzybiczych, które należy zastosować. W tym artykule dokonano przeglądu technik DNA i markerów molekularnych używanych do identyfikacji i charakteryzowania gatunków dermatofitów, a także aspektów ich filogenezy i ewolucji. Omówiono również zastosowania typowania szczepów molekularnych zarówno w badaniach podstawowych, jak i stosowanych (np. Taksonomia, ekologia, typowanie infekcji, wrażliwość na leki przeciwgrzybicze).
Protokół ten przedstawia metodę wycinania DNA lub RNA kluczowych organizmów zaangażowanych w określony proces biochemiczny w złożonym ekosystemie. Stabilne sondowanie izotopowe (SIP) umożliwia znakowanie i oddzielanie kwasów nukleinowych od członków społeczności, którzy są zaangażowani w ważne transformacje biochemiczne, ale często nie są najliczniejszymi członkami społeczności. Ta czysta, niezależna od kultury technika omija ograniczenia tradycyjnych technik izolacji drobnoustrojów lub eksploracji danych z szeroko zakrojonych badań metagenomicznych całej społeczności w celu ustalenia tożsamości i repertuaru genomowego mikroorganizmów uczestniczących w biologicznych cyklach składników odżywczych.
Eksperymenty SIP można zastosować w praktycznie każdym badanym ekosystemie i szlaku biochemicznym, pod warunkiem, że dostępny jest odpowiedni stabilny substrat izotopowy. Ta wszechstronna metodologia umożliwia przeprowadzanie szerokiego zakresu analiz, od analiz kwasów tłuszczowych, przez badania struktury społeczności i ekologii, po ukierunkowaną metagenomikę obejmującą sekwencjonowanie kwasów nukleinowych. Eksperymenty SIP stanowią skuteczną alternatywę dla szeroko zakrojonych badań metagenomicznych całej społeczności, ponieważ są ukierunkowane na organizmy lub transformacje biochemiczne będące przedmiotem zainteresowania, zmniejszając w ten sposób wysiłek związany z sekwencjonowaniem i czasochłonne analizy bioinformatyczne dużych zbiorów danych.
Analizy stabilnych izotopów i sygnaturowych kwasów tłuszczowych sugerują, że promienie manty rafowej żywią się zooplanktonem dennym.
Ocena troficznej roli i interakcji zwierzęcia jest kluczem do zrozumienia jego ogólnej ekologii i dynamiki. Konwencjonalne techniki stosowane do wyjaśnienia diety, takie jak analiza zawartości żołądka, nie są odpowiednie dla dużych zagrożonych gatunków morskich. Nieśmiercionośne pobieranie próbek w połączeniu z metodami biochemicznymi stanowi praktyczną alternatywę dla badania ekologii żerowania tych gatunków. Analiza stabilnych izotopów i sygnaturowych kwasów tłuszczowych tkanki mięśniowej została po raz pierwszy wykorzystana do zbadania zasymilowanej diety manty rafowej Manta alfredi i została porównana z różnymi grupami funkcjonalnymi zooplanktonu (tj. okresy karmienia, zooplankton epipelagiczny, zooplankton denny i kilka różnych taksonów zooplanktonu).
Stabilne wartości izotopu δ (15) N potwierdziły, że manty rafowe są konsumentami wtórnymi. Gatunek ten miał stosunkowo wysokie poziomy kwasu dokozaheksaenowego (DHA), co wskazuje na źródło pożywienia na bazie wiciowców w diecie, co prawdopodobnie odzwierciedla odżywianie się przybrzeżnym i epipelagicznym zooplanktonem bogatym w DHA. Jednak wysokie poziomy ω6 wielonienasyconych kwasów tłuszczowych i nieznacznie wzbogacone wartości δ (13) C w tkance manty rafowej sugerują, że nie żywią się one wyłącznie zooplanktonem pelagicznym, ale raczej pozyskują część swojej diety z innego pochodzenia.
Najbliższa zgodność dotyczyła zooplanktonu dennego, co sugeruje, że jest on ważnym składnikiem diety manty rafowej. Zdolność do odżywiania się zooplanktonem dennym jest prawdopodobnie związana z poziomymi i pionowymi wzorcami ruchu tego gigantycznego planktożercy. Te nowe informacje na temat użytkowania siedlisk i ekologii żywienia manty rafowej pomogą w skutecznej ocenie potrzeb w zakresie ochrony. Wartości podobieństwa obliczono przy użyciu prostego współczynnika dopasowania. Pięćdziesiąt dwa klastry o wielkości od 2 do 13 członków zostały zdefiniowane na poziomie podobieństwa 80-85% za pomocą ważonego algorytmu matematycznej średniej grupy par (WPGMA).
Gęstość, aktywność i różnorodność b akterii rodzimych dla krasowej warstwy wodonośnej.
Ekologia mikrobiologiczna krasowych wód podziemnych jest w dużej mierze nieznana. Gęstość, aktywność i różnorodność bakterii rodzimych dla podpowierzchniowego materiału krasowego w Parku Narodowym Mammoth Cave w Mammoth Cave w stanie Kentucky zbadano przy użyciu lokalnych procedur o minimalnym stopniu zakłócenia. Przebadano dwa stanowiska, położone 100 m pod powierzchnią ziemi, składające się z nasyconego drobnego do gruboziarnistego piasku w wodzie basenowej. Próbki pobierano aseptycznie przy użyciu zmodyfikowanych, jałowych strzykawek o pojemności 60 cm3.
Całkowitą gęstość komórek i całkowitą gęstość komórek oddychających określono przy użyciu procedury barwienia oranżem akrydyny / fioletem p-jodonitrotetrazoliowym (AO / INT). Komórki w wybranych rdzeniach wybarwiono INT i inkubowano w jaskini przez 4 godziny przed utrwaleniem aldehydem glutarowym, a następnie transportem do laboratorium. Komórki barwiono AO w laboratorium. Do określenia liczby żywych komórek zastosowano pożywki o niskiej i wysokiej zawartości składników odżywczych. Płytki inkubowano w jaskini przez 1 dzień w temperaturze otoczenia przed transportem do laboratorium w izolowanej chłodni. Liczba żywotnych komórek wahała się od 1,0 × 106 do 8,1 × 106 komórek na mokro g (-1) osadu. Całkowite bezpośrednie zliczenia wyniosły 3,9 x 106 i 1,4 x 107 komórek na mokro g (-1) odpowiednio dla miejsc Olivia’s Dome i Catherine’s Dome.
 Fluorescent labeled siRNA |
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| M1255-5 | Biovision | EUR 528 | |
Fluorescent labeled siRNA |
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| M1255-6 | Biovision | EUR 550 | |
Fluorescent labeled siRNA |
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| M1255-7 | Biovision | EUR 582 | |
Fluorescent labeled siRNA |
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| M1255-8 | Biovision | EUR 615 | |
Fluorescent labeled siRNA |
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| M1255-9 | Biovision | EUR 659 | |
 Human Mesenchymal Stem Cells-Green Fluorescent-Labeled |
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| SC00A2 | Neuromics | 500000 | EUR 962 |
 Human Mesenchymal Stem Cells-Red Fluorescent-Labeled |
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| SC00A3 | Neuromics | 500000 | EUR 962 |
 Labeled Neg Control siRNA |
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| M1257-1 | Biovision | EUR 311 | |
 Labeled siRNA Set E |
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| M1272-1 | Biovision | EUR 985 | |
 Labeled siRNA Set F |
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| M1273-1 | Biovision | EUR 1181 | |
 Fluorescent |
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| FPAK-3058--9K | Spherotech | 9X1 mL | EUR 563 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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 CXCR4-Phycoerythrin Labeled |
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| FC15004 | Neuromics | 100 Tests | EUR 448 |
 Ret-Fluorescein Labeled |
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| FC15016 | Neuromics | 100 Tests | EUR 448 |
 Ret-Allophycocyanin Labeled |
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| FC15017 | Neuromics | 100 Tests | EUR 448 |
 BDNF-Allophycocyanin Labeled |
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| FC15019 | Neuromics | 100 Tests | EUR 507 |
 , DNA Aptamer, FITC labeled) PCB72 (Aptamer, FITC labeled 9.3) , DNA Aptamer, FITC labeled |
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| AD-134-F | Alpha Diagnostics | Custom | Ask for price |
 Fluorescent Particles |
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| FH-10040-2 | Spherotech | 2 mL | EUR 259 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FH-10052-2 | Spherotech | 2 mL | EUR 259 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FH-10056-10 | Spherotech | 10 mL | EUR 852 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FH-10056-2 | Spherotech | 2 mL | EUR 259 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FH-10062-2 | Spherotech | 2 mL | EUR 259 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FH-10074-2 | Spherotech | 2 mL | EUR 259 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FH-10078-2 | Spherotech | 2 mL | EUR 259 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FH-2040-2 | Spherotech | 2 mL | EUR 192 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FH-2045-2 | Spherotech | 2 mL | EUR 192 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FH-2052-2 | Spherotech | 2 mL | EUR 192 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FH-2056-2 | Spherotech | 2 mL | EUR 192 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FH-2060-2 | Spherotech | 2 mL | EUR 218 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FH-2062-2 | Spherotech | 2 mL | EUR 192 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FH-2070-2 | Spherotech | 2 mL | EUR 192 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FH-3056-10 | Spherotech | 10 mL | EUR 669 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FH-3056-2 | Spherotech | 2 mL | EUR 198 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FH-5056-2 | Spherotech | 2 mL | EUR 198 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FL-0845-2 | Spherotech | 2 mL | EUR 233 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FL-0852-2 | Spherotech | 2 mL | EUR 188 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FL-10056-2 | Spherotech | 2 mL | EUR 259 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FL-10078-2 | Spherotech | 2 mL | EUR 259 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FL-2040-2 | Spherotech | 2 mL | EUR 192 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FL-2045-2 | Spherotech | 2 mL | EUR 192 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FL-2052-2 | Spherotech | 2 mL | EUR 192 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FL-2056-2 | Spherotech | 2 mL | EUR 192 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FL-2060-2 | Spherotech | 2 mL | EUR 218 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FL-2062-2 | Spherotech | 2 mL | EUR 192 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FL-2068-2 | Spherotech | 2 mL | EUR 192 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FL-3068-2 | Spherotech | 2 mL | EUR 198 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FL-4070-2 | Spherotech | 2 mL | EUR 198 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FL-6056-2 | Spherotech | 2 mL | EUR 218 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FL-6068-2 | Spherotech | 2 mL | EUR 218 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-00552-2 | Spherotech | 2 mL | EUR 182 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-00556-2 | Spherotech | 2 mL | EUR 182 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-00558-2 | Spherotech | 2 mL | EUR 182 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-00562-2 | Spherotech | 2 mL | EUR 182 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-00570-2 | Spherotech | 2 mL | EUR 182 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-0245-2 | Spherotech | 2 mL | EUR 182 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-0252-2 | Spherotech | 2 mL | EUR 182 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-0256-2 | Spherotech | 2 mL | EUR 182 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-0257-2 | Spherotech | 2 mL | EUR 198 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-0262-2 | Spherotech | 2 mL | EUR 182 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-0270-2 | Spherotech | 2 mL | EUR 182 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-0545-2 | Spherotech | 2 mL | EUR 182 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-0552-2 | Spherotech | 2 mL | EUR 182 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-0556-2 | Spherotech | 2 mL | EUR 182 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-0557-2 | Spherotech | 2 mL | EUR 198 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-0558-2 | Spherotech | 2 mL | EUR 182 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-0562-2 | Spherotech | 2 mL | EUR 182 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-0570-2 | Spherotech | 2 mL | EUR 182 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-0842-2 | Spherotech | 2 mL | EUR 188 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-0845-2 | Spherotech | 2 mL | EUR 188 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-0852-2 | Spherotech | 2 mL | EUR 188 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-0856-2 | Spherotech | 2 mL | EUR 188 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-0857-2 | Spherotech | 2 mL | EUR 202 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-0858-2 | Spherotech | 2 mL | EUR 188 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-0862-2 | Spherotech | 2 mL | EUR 188 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-0868-2 | Spherotech | 2 mL | EUR 188 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-0870-2 | Spherotech | 2 mL | EUR 188 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-0878-2 | Spherotech | 2 mL | EUR 188 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-10040-2 | Spherotech | 2 mL | EUR 259 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-10045-2 | Spherotech | 2 mL | EUR 259 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-10052-2 | Spherotech | 2 mL | EUR 259 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-10056-10 | Spherotech | 10 mL | EUR 740 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-10056-2 | Spherotech | 2 mL | EUR 259 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-10056-2A | Spherotech | 2X1 mL | EUR 259 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-10062-2 | Spherotech | 2 mL | EUR 259 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-10066-2 | Spherotech | 2 mL | EUR 259 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-10067-2 | Spherotech | 2 mL | EUR 259 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-10070-2 | Spherotech | 2 mL | EUR 259 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-10074-2 | Spherotech | 2 mL | EUR 259 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-10078-2 | Spherotech | 2 mL | EUR 259 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-15052-2 | Spherotech | 2 mL | EUR 283 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-15056-2 | Spherotech | 2 mL | EUR 283 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-15062-2 | Spherotech | 2 mL | EUR 283 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-15066-2 | Spherotech | 2 mL | EUR 283 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-15070-2 | Spherotech | 2 mL | EUR 283 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-1552-2 | Spherotech | 2 mL | EUR 202 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-20052-5 | Spherotech | 5 mL | EUR 283 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-20056-5 | Spherotech | 5 mL | EUR 283 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-2042-2 | Spherotech | 2 mL | EUR 218 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-2045-2 | Spherotech | 2 mL | EUR 192 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-2054-2 | Spherotech | 2 mL | EUR 192 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-2056-2 | Spherotech | 2 mL | EUR 192 |
|
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-2057-2 | Spherotech | 2 mL | EUR 198 |
|
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-2058-2 | Spherotech | 2 mL | EUR 192 |
|
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-2060-2 | Spherotech | 2 mL | EUR 218 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-2062-2 | Spherotech | 2 mL | EUR 192 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-2065-2 | Spherotech | 2 mL | EUR 192 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-2068-2 | Spherotech | 2 mL | EUR 192 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-2070-2 | Spherotech | 2 mL | EUR 192 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-30052-5 | Spherotech | 5 mL | EUR 283 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-30056-5 | Spherotech | 5 mL | EUR 283 |
|
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-30062-5 | Spherotech | 5 mL | EUR 283 |
|
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-3042-2 | Spherotech | 2 mL | EUR 218 |
|
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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 Fluorescent Particle |
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| FP-3045-5 | Spherotech | 5 mL | EUR 253 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-3052-2 | Spherotech | 2 mL | EUR 198 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-3055-2 | Spherotech | 2 mL | EUR 218 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-3056-2 | Spherotech | 2 mL | EUR 198 |
|
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-3057-2 | Spherotech | 2 mL | EUR 198 |
|
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-3058-2 | Spherotech | 2 mL | EUR 198 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-3065-2 | Spherotech | 2 mL | EUR 198 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-3066-2 | Spherotech | 2 mL | EUR 218 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-3068-2 | Spherotech | 2 mL | EUR 198 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-3069-2 | Spherotech | 2 mL | EUR 198 |
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Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-3070-2 | Spherotech | 2 mL | EUR 198 |
|
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-3074-2 | Spherotech | 2 mL | EUR 202 |
|
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-3078-2 | Spherotech | 2 mL | EUR 202 |
|
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-3079-2 | Spherotech | 2 mL | EUR 202 |
|
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-4052-2 | Spherotech | 2 mL | EUR 198 |
|
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-4056-2 | Spherotech | 2 mL | EUR 198 |
|
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-4058-2 | Spherotech | 2 mL | EUR 198 |
|
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-4060-2 | Spherotech | 2 mL | EUR 218 |
|
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-4062-2 | Spherotech | 2 mL | EUR 198 |
|
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-4070-2 | Spherotech | 2 mL | EUR 198 |
|
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-5056-10 | Spherotech | 10 mL | EUR 740 |
|
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-5066-2 | Spherotech | 2 mL | EUR 218 |
|
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-5074-2 | Spherotech | 2 mL | EUR 202 |
|
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
|||
Fluorescent Particles |
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| FP-5078-2 | Spherotech | 2 mL | EUR 202 |
|
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-6052-2 | Spherotech | 2 mL | EUR 218 |
|
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-6056-2 | Spherotech | 2 mL | EUR 218 |
|
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-6057-2 | Spherotech | 2 mL | EUR 218 |
|
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Fluorescent Particles |
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| FP-6058-2 | Spherotech | 2 mL | EUR 218 |
|
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
|||
Fluorescent Particles |
|||
| FP-6069-2 | Spherotech | 2 mL | EUR 218 |
|
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
|||
Fluorescent Particles |
|||
| FP-7052-2 | Spherotech | 2 mL | EUR 218 |
|
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
|||
Fluorescent Particles |
|||
| FP-7070-2 | Spherotech | 2 mL | EUR 218 |
|
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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 Annexin V, FITC Labeled |
|||
| 20030 | AAT Bioquest | 100 tests | EUR 219 |
 FGF R3-Fluorescein Labeled |
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| FC15006 | Neuromics | 100 Tests | EUR 448 |
 gp130/CD130-Phycoerythrin Labeled |
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| FC15014 | Neuromics | 100 Tests | EUR 448 |
 FGF-R2-Allophycocyanin Labeled |
|||
| FC15015 | Neuromics | 100 Tests | EUR 448 |
 Fluorescent sensor, ANQ |
|||
| 9695-25 | Biovision | EUR 675 | |
Fluorescent sensor, ANQ |
|||
| 9695-5 | Biovision | EUR 207 | |
 Cyan Fluorescent Protein |
|||
| 30R-2808 | Fitzgerald | 100 ug | EUR 527 |
|
Description: Purified recombinant Human Cyan Fluorescent Protein |
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 dsRed Fluorescent protein |
|||
| 30R-2809 | Fitzgerald | 100 ug | EUR 491 |
|
Description: Purified recombinant Human dsRed Fluorescent protein |
|||
 mCherry Fluorescent Protein |
|||
| 4993-100 | Biovision | EUR 408 | |
mCherry Fluorescent Protein |
|||
| 4993-1000 | Biovision | EUR 2621 | |
mCherry Fluorescent Protein |
|||
| 4993-5000 | Biovision | EUR 7646 | |
Liczba żywych komórek była bardzo podobna do liczby komórek oddychających w obu ośrodkach. W miejscu Olivia’s Dome liczba żywych komórek stanowiła 26-31% bezpośrednich zliczeń komórek, podczas gdy 58% całkowitej liczby komórek aktywnie oddychało. W miejscu Catherine’s Dome liczba żywych komórek stanowiła 11-58% bezpośrednich zliczeń, podczas gdy 53% komórek aktywnie oddychało. Łącznie 237 szczepów odzyskanych z pożywek o niskiej i wysokiej zawartości składników odżywczych zarówno w Olivia’s, jak i Catherine’s Domes, a 10 szczepów referencyjnych zbadano pod kątem 117 cech morfologicznych, biochemicznych i fizjologicznych. Wyniki zakodowano binarnie i przeanalizowano za pomocą numerycznych technik taksonomicznych. Macierz została zbadana przy użyciu współczynnika Jaccarda i grupowania WPGMA w celu kontroli zniekształceń spowodowanych negatywnymi dopasowaniami i różną wielkością grupy.
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