Protein-protein interactions between two proteins have generally been studied using biochemical techniques such as crosslinking, co-immunoprecipitation and co-fractionation by chromatography. We have generated a novel genetic system to study these interactions by taking advantage of the properties of the GAL4 protein of the yeast Saccharomyces cerevisiae.

This protein is a transcriptional activator required for the expression of genes encoding enzymes of galactose utilization. It consists of two separable and functionally essential domains: an N-terminal domain which binds to specific DNA sequences (UASG); and a C-terminal domain containing acidic regions, which is necessary to activate transcription.

Caspases Group II, Activ Human
K242-3-25	Biovision	each	EUR 483.6

Active Human Caspases Set III
K232-8-25	Biovision	each	EUR 1005.6

Active Human Caspases Group IV
K2060-250	ApexBio	10x25 units	EUR 1470

Magic Red™ Caspases 3&7
KF17306	Neuromics	25 Tests	EUR 342

Magic Red™ Caspases 3&7
KF17307	Neuromics	100 Tests	EUR 834

Active Human Caspases Group III
K2063-100	ApexBio	4x25 units	EUR 769.2

Caspases Group III, Active Human
K243-4-25	Biovision	each	EUR 614.4

Human Caspases 3/7 Elisa Kit
QY-E05405	Qayee Biotechnology	96T	EUR 433.2

Active Human Caspases Group I, Active Human
K241-3-25	Biovision	each	EUR 444

WHATMAN CELLULOSE EXTRACTION THIMBLE DO
WHA2810432	Scientific Laboratory Supplies	PK25	EUR 270

DO Edge Multi with DO Probe
PHM0344	Scientific Laboratory Supplies	EACH	EUR 1086

DO membranes
PHM0360	Scientific Laboratory Supplies	PK5	EUR 136.8

DO-264
HY-114157	MedChemExpress	50mg	EUR 1299.6

DO-264
T11069-10mg	TargetMol Chemicals	10mg	Ask for price
Description: DO-264

DO-264
T11069-1g	TargetMol Chemicals	1g	Ask for price
Description: DO-264

DO-264
T11069-1mg	TargetMol Chemicals	1mg	Ask for price
Description: DO-264

DO-264
T11069-50mg	TargetMol Chemicals	50mg	Ask for price
Description: DO-264

DO-264
T11069-5mg	TargetMol Chemicals	5mg	Ask for price
Description: DO-264

DO-601
T31559-10mg	TargetMol Chemicals	10mg	Ask for price
Description: DO-601

DO-601
T31559-1g	TargetMol Chemicals	1g	Ask for price
Description: DO-601

DO-601
T31559-1mg	TargetMol Chemicals	1mg	Ask for price
Description: DO-601

DO-601
T31559-50mg	TargetMol Chemicals	50mg	Ask for price
Description: DO-601

DO-601
T31559-5mg	TargetMol Chemicals	5mg	Ask for price
Description: DO-601

DO probe electrolyte
DIS0024	Scientific Laboratory Supplies	EACH	EUR 45.6

p53(DO-7) Antibody
BNCA0513-250	Biotium	250uL	EUR 459.6
Description: Primary antibody against p53(DO-7), APC conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody
BNC800513-100	Biotium	100uL	EUR 238.8
Description: Primary antibody against p53(DO-7), CF680 conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody
BNC800513-500	Biotium	500uL	EUR 652.8
Description: Primary antibody against p53(DO-7), CF680 conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody
BNC810513-100	Biotium	100uL	EUR 238.8
Description: Primary antibody against p53(DO-7), CF680R conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody
BNC810513-500	Biotium	500uL	EUR 652.8
Description: Primary antibody against p53(DO-7), CF680R conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody
BNC940513-100	Biotium	100uL	EUR 238.8
Description: Primary antibody against p53(DO-7), CF594 conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody
BNC940513-500	Biotium	500uL	EUR 652.8
Description: Primary antibody against p53(DO-7), CF594 conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody
BNC700513-100	Biotium	100uL	EUR 238.8
Description: Primary antibody against p53(DO-7), CF770 conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody
BNC700513-500	Biotium	500uL	EUR 652.8
Description: Primary antibody against p53(DO-7), CF770 conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody
BNC880513-100	Biotium	100uL	EUR 238.8
Description: Primary antibody against p53(DO-7), CF488A conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody
BNC880513-500	Biotium	500uL	EUR 652.8
Description: Primary antibody against p53(DO-7), CF488A conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody
BNC400513-100	Biotium	100uL	EUR 238.8
Description: Primary antibody against p53(DO-7), CF640R conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody
BNC400513-500	Biotium	500uL	EUR 652.8
Description: Primary antibody against p53(DO-7), CF640R conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody
BNC050513-100	Biotium	100uL	EUR 238.8
Description: Primary antibody against p53(DO-7), CF405M conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody
BNC050513-500	Biotium	500uL	EUR 652.8
Description: Primary antibody against p53(DO-7), CF405M conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody
BNCB0513-100	Biotium	100uL	EUR 238.8
Description: Primary antibody against p53(DO-7), Biotin conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody
BNCB0513-500	Biotium	500uL	EUR 652.8
Description: Primary antibody against p53(DO-7), Biotin conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody
BNCAP0513-100	Biotium	100uL	EUR 238.8
Description: Primary antibody against p53(DO-7), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody
BNCAP0513-500	Biotium	500uL	EUR 652.8
Description: Primary antibody against p53(DO-7), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody
BNCR0513-250	Biotium	250uL	EUR 459.6
Description: Primary antibody against p53(DO-7), RPE conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody
BNCP0513-250	Biotium	250uL	EUR 459.6
Description: Primary antibody against p53(DO-7), PerCP conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody
BNUM0513-50	Biotium	50uL	EUR 474
Description: Primary antibody against p53(DO-7), 1mg/mL

p53(DO-7) Antibody
BNCH0513-100	Biotium	100uL	EUR 238.8
Description: Primary antibody against p53(DO-7), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody
BNCH0513-500	Biotium	500uL	EUR 652.8
Description: Primary antibody against p53(DO-7), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody
BNUB0513-100	Biotium	100uL	EUR 250.8
Description: Primary antibody against p53(DO-7), Concentration: 0.2mg/mL

p53(DO-7) Antibody
BNUB0513-500	Biotium	500uL	EUR 549.6
Description: Primary antibody against p53(DO-7), Concentration: 0.2mg/mL

p53(DO-7) Antibody
BNC040513-100	Biotium	100uL	EUR 238.8
Description: Primary antibody against p53(DO-7), CF405S conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody
BNC040513-500	Biotium	500uL	EUR 652.8
Description: Primary antibody against p53(DO-7), CF405S conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody
BNC550513-100	Biotium	100uL	EUR 238.8
Description: Primary antibody against p53(DO-7), CF555 conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody
BNC550513-500	Biotium	500uL	EUR 652.8
Description: Primary antibody against p53(DO-7), CF555 conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody
BNC610513-100	Biotium	100uL	EUR 238.8
Description: Primary antibody against p53(DO-7), CF660R conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody
BNC610513-500	Biotium	500uL	EUR 652.8
Description: Primary antibody against p53(DO-7), CF660R conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody
BNC680513-100	Biotium	100uL	EUR 238.8
Description: Primary antibody against p53(DO-7), CF568 conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody
BNC680513-500	Biotium	500uL	EUR 652.8
Description: Primary antibody against p53(DO-7), CF568 conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody
BNC470513-100	Biotium	100uL	EUR 238.8
Description: Primary antibody against p53(DO-7), CF647 conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody
BNC470513-500	Biotium	500uL	EUR 652.8
Description: Primary antibody against p53(DO-7), CF647 conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody
BNC430513-100	Biotium	100uL	EUR 238.8
Description: Primary antibody against p53(DO-7), CF543 conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody
BNC430513-500	Biotium	500uL	EUR 652.8
Description: Primary antibody against p53(DO-7), CF543 conjugate, Concentration: 0.1mg/mL

LE621 IP67 DO Electrode
DIS1093	Scientific Laboratory Supplies	EACH	EUR 445.2

HLA-DO? Polyclonal Antibody
ES2536-100ul	ELK Biotech	100ul	EUR 334.8
Description: A Rabbit Polyclonal antibody against HLA-DO? from Human. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA

HLA-DO? Polyclonal Antibody
ES2536-50ul	ELK Biotech	50ul	EUR 248.4
Description: A Rabbit Polyclonal antibody against HLA-DO? from Human. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA

HLA-DO? Polyclonal Antibody
ES2537-100ul	ELK Biotech	100ul	EUR 334.8
Description: A Rabbit Polyclonal antibody against HLA-DO? from Human. This antibody is tested and validated for WB, ELISA, WB, ELISA

HLA-DO? Polyclonal Antibody
ES2537-50ul	ELK Biotech	50ul	EUR 248.4
Description: A Rabbit Polyclonal antibody against HLA-DO? from Human. This antibody is tested and validated for WB, ELISA, WB, ELISA

DO probe with 10m cable
DIS1024	Scientific Laboratory Supplies	EACH	EUR 428.4

DO probe with 4m cable
DIS1026	Scientific Laboratory Supplies	EACH	EUR 330

FiveGo F4 DO F4-Meter
DIS1090	Scientific Laboratory Supplies	EACH	EUR 472.8

FiveGo F4 DO F4-Standard
DIS1091	Scientific Laboratory Supplies	EACH	EUR 882

FiveGo F4 DO F4-Field
DIS1092	Scientific Laboratory Supplies	EACH	EUR 945.6

Seven2Go S4 pol DO meter
PHM0180	Scientific Laboratory Supplies	EACH	EUR 626.4

Seven2Go S9 opt DO meter
PHM0183	Scientific Laboratory Supplies	EACH	EUR 1034.4

Wproof DO meter with BOD
PHM0358	Scientific Laboratory Supplies	EACH	EUR 1131.6

Sign 100X75 Do Not Drink
P04CS	Scientific Laboratory Supplies	EACH	EUR 2.63

p53(BP53-12 + DO-7) Antibody
BNCA0723-250	Biotium	250uL	EUR 459.6
Description: Primary antibody against p53(BP53-12 + DO-7), APC conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody
BNC800723-100	Biotium	100uL	EUR 238.8
Description: Primary antibody against p53(BP53-12 + DO-7), CF680 conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody
BNC800723-500	Biotium	500uL	EUR 652.8
Description: Primary antibody against p53(BP53-12 + DO-7), CF680 conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody
BNC810723-100	Biotium	100uL	EUR 238.8
Description: Primary antibody against p53(BP53-12 + DO-7), CF680R conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody
BNC810723-500	Biotium	500uL	EUR 652.8
Description: Primary antibody against p53(BP53-12 + DO-7), CF680R conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody
BNC940723-100	Biotium	100uL	EUR 238.8
Description: Primary antibody against p53(BP53-12 + DO-7), CF594 conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody
BNC940723-500	Biotium	500uL	EUR 652.8
Description: Primary antibody against p53(BP53-12 + DO-7), CF594 conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody
BNC700723-100	Biotium	100uL	EUR 238.8
Description: Primary antibody against p53(BP53-12 + DO-7), CF770 conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody
BNC700723-500	Biotium	500uL	EUR 652.8
Description: Primary antibody against p53(BP53-12 + DO-7), CF770 conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody
BNC880723-100	Biotium	100uL	EUR 238.8
Description: Primary antibody against p53(BP53-12 + DO-7), CF488A conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody
BNC880723-500	Biotium	500uL	EUR 652.8
Description: Primary antibody against p53(BP53-12 + DO-7), CF488A conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody
BNC050723-100	Biotium	100uL	EUR 238.8
Description: Primary antibody against p53(BP53-12 + DO-7), CF405M conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody
BNC050723-500	Biotium	500uL	EUR 652.8
Description: Primary antibody against p53(BP53-12 + DO-7), CF405M conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody
BNC040723-100	Biotium	100uL	EUR 238.8
Description: Primary antibody against p53(BP53-12 + DO-7), CF405S conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody
BNC040723-500	Biotium	500uL	EUR 652.8
Description: Primary antibody against p53(BP53-12 + DO-7), CF405S conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody
BNC400723-100	Biotium	100uL	EUR 238.8
Description: Primary antibody against p53(BP53-12 + DO-7), CF640R conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody
BNC400723-500	Biotium	500uL	EUR 652.8
Description: Primary antibody against p53(BP53-12 + DO-7), CF640R conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody
BNCB0723-100	Biotium	100uL	EUR 238.8
Description: Primary antibody against p53(BP53-12 + DO-7), Biotin conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody
BNCB0723-500	Biotium	500uL	EUR 652.8
Description: Primary antibody against p53(BP53-12 + DO-7), Biotin conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody
BNCAP0723-100	Biotium	100uL	EUR 238.8
Description: Primary antibody against p53(BP53-12 + DO-7), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody
BNCAP0723-500	Biotium	500uL	EUR 652.8
Description: Primary antibody against p53(BP53-12 + DO-7), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody
BNCR0723-250	Biotium	250uL	EUR 459.6
Description: Primary antibody against p53(BP53-12 + DO-7), RPE conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody
BNCP0723-250	Biotium	250uL	EUR 459.6
Description: Primary antibody against p53(BP53-12 + DO-7), PerCP conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody
BNUM0723-50	Biotium	50uL	EUR 474
Description: Primary antibody against p53(BP53-12 + DO-7), 1mg/mL

p53(BP53-12 + DO-7) Antibody
BNCH0723-100	Biotium	100uL	EUR 238.8
Description: Primary antibody against p53(BP53-12 + DO-7), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody
BNCH0723-500	Biotium	500uL	EUR 652.8
Description: Primary antibody against p53(BP53-12 + DO-7), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody
BNUB0723-100	Biotium	100uL	EUR 250.8
Description: Primary antibody against p53(BP53-12 + DO-7), Concentration: 0.2mg/mL

p53(BP53-12 + DO-7) Antibody
BNUB0723-500	Biotium	500uL	EUR 549.6
Description: Primary antibody against p53(BP53-12 + DO-7), Concentration: 0.2mg/mL

p53(BP53-12 + DO-7) Antibody
BNC550723-100	Biotium	100uL	EUR 238.8
Description: Primary antibody against p53(BP53-12 + DO-7), CF555 conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody
BNC550723-500	Biotium	500uL	EUR 652.8
Description: Primary antibody against p53(BP53-12 + DO-7), CF555 conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody
BNC610723-100	Biotium	100uL	EUR 238.8
Description: Primary antibody against p53(BP53-12 + DO-7), CF660R conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody
BNC610723-500	Biotium	500uL	EUR 652.8
Description: Primary antibody against p53(BP53-12 + DO-7), CF660R conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody
BNC470723-100	Biotium	100uL	EUR 238.8
Description: Primary antibody against p53(BP53-12 + DO-7), CF647 conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody
BNC470723-500	Biotium	500uL	EUR 652.8
Description: Primary antibody against p53(BP53-12 + DO-7), CF647 conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody
BNC430723-100	Biotium	100uL	EUR 238.8
Description: Primary antibody against p53(BP53-12 + DO-7), CF543 conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody
BNC430723-500	Biotium	500uL	EUR 652.8
Description: Primary antibody against p53(BP53-12 + DO-7), CF543 conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody
BNC680723-100	Biotium	100uL	EUR 238.8
Description: Primary antibody against p53(BP53-12 + DO-7), CF568 conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody
BNC680723-500	Biotium	500uL	EUR 652.8
Description: Primary antibody against p53(BP53-12 + DO-7), CF568 conjugate, Concentration: 0.1mg/mL

Sign 210x148mm Do Not Drink R..
PH00251R	Scientific Laboratory Supplies	EACH	EUR 10.44

Sign 210x148mm Do Not Use Mob..
PH01051R	Scientific Laboratory Supplies	EACH	EUR 10.44

Sign 210x148mm Do Not Use Mob..
PH01051S	Scientific Laboratory Supplies	EACH	EUR 6.53

p53; Clone DO-1 (Ready-To-Use)
A20027-0002	ScyTek Laboratories	2 ml	EUR 100.8

p53; Clone DO-7 (Ready-To-Use)
A00021-0002	ScyTek Laboratories	2 ml	EUR 201.6

p53; Clone DO-7 (Ready-To-Use)
A00021-0007	ScyTek Laboratories	7 ml	EUR 344.4

p53; Clone DO-7 (Ready-To-Use)
A00021-0025	ScyTek Laboratories	25 ml	EUR 1058.4

p53; Clone DO-1 (Ready-To-Use)
A00027-0007	ScyTek Laboratories	7 ml	EUR 139.2

p53; Clone DO-1 (Ready-To-Use)
A00027-0025	ScyTek Laboratories	25 ml	EUR 336

We have generated a system of two hybrid proteins containing parts of GAL4: the GAL4 DNA-binding domain fused to a protein ‘X’ and a GAL4 activating region fused to a protein ‘Y’. If X and Y can form a protein-protein complex and reconstitute proximity of the GAL4 domains, transcription of a gene regulated by UASG occurs.

We have tested this system using two yeast proteins that are known to interact–SNF1 and SNF4. High transcriptional activity is obtained only when both hybrids are present in a cell. This system may be applicable as a general method to identify proteins that interact with a known protein by the use of a simple galactose selection.