A novel genetic system to detect protein-protein interactions.

Protein-protein interactions between two proteins have generally been studied using biochemical techniques such as crosslinking, co-immunoprecipitation and co-fractionation by chromatography. We have generated a novel genetic system to study these interactions by taking advantage of the properties of the GAL4 protein of the yeast Saccharomyces cerevisiae.

This protein is a transcriptional activator required for the expression of genes encoding enzymes of galactose utilization. It consists of two separable and functionally essential domains: an N-terminal domain which binds to specific DNA sequences (UASG); and a C-terminal domain containing acidic regions, which is necessary to activate transcription.

HLA-DO? Polyclonal Antibody

ES2537-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against HLA-DO? from Human. This antibody is tested and validated for WB, ELISA, WB, ELISA

p53(DO-7) Antibody

BNCA0513-250 250uL
EUR 383
Description: Primary antibody against p53(DO-7), APC conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody

BNCAP0513-100 100uL
EUR 199
Description: Primary antibody against p53(DO-7), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody

BNCAP0513-500 500uL
EUR 544
Description: Primary antibody against p53(DO-7), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody

BNC810513-100 100uL
EUR 199
Description: Primary antibody against p53(DO-7), CF680R conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody

BNC810513-500 500uL
EUR 544
Description: Primary antibody against p53(DO-7), CF680R conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody

BNC880513-100 100uL
EUR 199
Description: Primary antibody against p53(DO-7), CF488A conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody

BNC880513-500 500uL
EUR 544
Description: Primary antibody against p53(DO-7), CF488A conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody

BNCB0513-100 100uL
EUR 199
Description: Primary antibody against p53(DO-7), Biotin conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody

BNCB0513-500 500uL
EUR 544
Description: Primary antibody against p53(DO-7), Biotin conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody

BNC550513-100 100uL
EUR 199
Description: Primary antibody against p53(DO-7), CF555 conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody

BNC550513-500 500uL
EUR 544
Description: Primary antibody against p53(DO-7), CF555 conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody

BNC430513-100 100uL
EUR 199
Description: Primary antibody against p53(DO-7), CF543 conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody

BNC430513-500 500uL
EUR 544
Description: Primary antibody against p53(DO-7), CF543 conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody

BNC470513-100 100uL
EUR 199
Description: Primary antibody against p53(DO-7), CF647 conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody

BNC470513-500 500uL
EUR 544
Description: Primary antibody against p53(DO-7), CF647 conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody

BNC940513-100 100uL
EUR 199
Description: Primary antibody against p53(DO-7), CF594 conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody

BNC940513-500 500uL
EUR 544
Description: Primary antibody against p53(DO-7), CF594 conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody

BNCP0513-250 250uL
EUR 383
Description: Primary antibody against p53(DO-7), PerCP conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody

BNC040513-100 100uL
EUR 199
Description: Primary antibody against p53(DO-7), CF405S conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody

BNC040513-500 500uL
EUR 544
Description: Primary antibody against p53(DO-7), CF405S conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody

BNC050513-100 100uL
EUR 199
Description: Primary antibody against p53(DO-7), CF405M conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody

BNC050513-500 500uL
EUR 544
Description: Primary antibody against p53(DO-7), CF405M conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody

BNCR0513-250 250uL
EUR 383
Description: Primary antibody against p53(DO-7), RPE conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody

BNUB0513-100 100uL
EUR 209
Description: Primary antibody against p53(DO-7), Concentration: 0.2mg/mL

p53(DO-7) Antibody

BNUB0513-500 500uL
EUR 458
Description: Primary antibody against p53(DO-7), Concentration: 0.2mg/mL

p53(DO-7) Antibody

BNC680513-100 100uL
EUR 199
Description: Primary antibody against p53(DO-7), CF568 conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody

BNC680513-500 500uL
EUR 544
Description: Primary antibody against p53(DO-7), CF568 conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody

BNC700513-100 100uL
EUR 199
Description: Primary antibody against p53(DO-7), CF770 conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody

BNC700513-500 500uL
EUR 544
Description: Primary antibody against p53(DO-7), CF770 conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody

BNC800513-100 100uL
EUR 199
Description: Primary antibody against p53(DO-7), CF680 conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody

BNC800513-500 500uL
EUR 544
Description: Primary antibody against p53(DO-7), CF680 conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody

BNC400513-100 100uL
EUR 199
Description: Primary antibody against p53(DO-7), CF640R conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody

BNC400513-500 500uL
EUR 544
Description: Primary antibody against p53(DO-7), CF640R conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody

BNC610513-100 100uL
EUR 199
Description: Primary antibody against p53(DO-7), CF660R conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody

BNC610513-500 500uL
EUR 544
Description: Primary antibody against p53(DO-7), CF660R conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody

BNCH0513-100 100uL
EUR 199
Description: Primary antibody against p53(DO-7), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody

BNCH0513-500 500uL
EUR 544
Description: Primary antibody against p53(DO-7), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

p53(DO-7) Antibody

BNUM0513-50 50uL
EUR 395
Description: Primary antibody against p53(DO-7), 1mg/mL

p53(BP53-12 + DO-7) Antibody

BNCA0723-250 250uL
EUR 383
Description: Primary antibody against p53(BP53-12 + DO-7), APC conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody

BNCAP0723-100 100uL
EUR 199
Description: Primary antibody against p53(BP53-12 + DO-7), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody

BNCAP0723-500 500uL
EUR 544
Description: Primary antibody against p53(BP53-12 + DO-7), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody

BNC810723-100 100uL
EUR 199
Description: Primary antibody against p53(BP53-12 + DO-7), CF680R conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody

BNC810723-500 500uL
EUR 544
Description: Primary antibody against p53(BP53-12 + DO-7), CF680R conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody

BNC880723-100 100uL
EUR 199
Description: Primary antibody against p53(BP53-12 + DO-7), CF488A conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody

BNC880723-500 500uL
EUR 544
Description: Primary antibody against p53(BP53-12 + DO-7), CF488A conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody

BNCB0723-100 100uL
EUR 199
Description: Primary antibody against p53(BP53-12 + DO-7), Biotin conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody

BNCB0723-500 500uL
EUR 544
Description: Primary antibody against p53(BP53-12 + DO-7), Biotin conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody

BNC550723-100 100uL
EUR 199
Description: Primary antibody against p53(BP53-12 + DO-7), CF555 conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody

BNC550723-500 500uL
EUR 544
Description: Primary antibody against p53(BP53-12 + DO-7), CF555 conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody

BNC430723-100 100uL
EUR 199
Description: Primary antibody against p53(BP53-12 + DO-7), CF543 conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody

BNC430723-500 500uL
EUR 544
Description: Primary antibody against p53(BP53-12 + DO-7), CF543 conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody

BNC470723-100 100uL
EUR 199
Description: Primary antibody against p53(BP53-12 + DO-7), CF647 conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody

BNC470723-500 500uL
EUR 544
Description: Primary antibody against p53(BP53-12 + DO-7), CF647 conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody

BNC940723-100 100uL
EUR 199
Description: Primary antibody against p53(BP53-12 + DO-7), CF594 conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody

BNC940723-500 500uL
EUR 544
Description: Primary antibody against p53(BP53-12 + DO-7), CF594 conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody

BNCP0723-250 250uL
EUR 383
Description: Primary antibody against p53(BP53-12 + DO-7), PerCP conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody

BNC040723-100 100uL
EUR 199
Description: Primary antibody against p53(BP53-12 + DO-7), CF405S conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody

BNC040723-500 500uL
EUR 544
Description: Primary antibody against p53(BP53-12 + DO-7), CF405S conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody

BNC050723-100 100uL
EUR 199
Description: Primary antibody against p53(BP53-12 + DO-7), CF405M conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody

BNC050723-500 500uL
EUR 544
Description: Primary antibody against p53(BP53-12 + DO-7), CF405M conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody

BNCR0723-250 250uL
EUR 383
Description: Primary antibody against p53(BP53-12 + DO-7), RPE conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody

BNUB0723-100 100uL
EUR 209
Description: Primary antibody against p53(BP53-12 + DO-7), Concentration: 0.2mg/mL

p53(BP53-12 + DO-7) Antibody

BNUB0723-500 500uL
EUR 458
Description: Primary antibody against p53(BP53-12 + DO-7), Concentration: 0.2mg/mL

p53(BP53-12 + DO-7) Antibody

BNC680723-100 100uL
EUR 199
Description: Primary antibody against p53(BP53-12 + DO-7), CF568 conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody

BNC680723-500 500uL
EUR 544
Description: Primary antibody against p53(BP53-12 + DO-7), CF568 conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody

BNC700723-100 100uL
EUR 199
Description: Primary antibody against p53(BP53-12 + DO-7), CF770 conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody

BNC700723-500 500uL
EUR 544
Description: Primary antibody against p53(BP53-12 + DO-7), CF770 conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody

BNC400723-100 100uL
EUR 199
Description: Primary antibody against p53(BP53-12 + DO-7), CF640R conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody

BNC400723-500 500uL
EUR 544
Description: Primary antibody against p53(BP53-12 + DO-7), CF640R conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody

BNC610723-100 100uL
EUR 199
Description: Primary antibody against p53(BP53-12 + DO-7), CF660R conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody

BNC610723-500 500uL
EUR 544
Description: Primary antibody against p53(BP53-12 + DO-7), CF660R conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody

BNC800723-100 100uL
EUR 199
Description: Primary antibody against p53(BP53-12 + DO-7), CF680 conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody

BNC800723-500 500uL
EUR 544
Description: Primary antibody against p53(BP53-12 + DO-7), CF680 conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody

BNCH0723-100 100uL
EUR 199
Description: Primary antibody against p53(BP53-12 + DO-7), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody

BNCH0723-500 500uL
EUR 544
Description: Primary antibody against p53(BP53-12 + DO-7), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

p53(BP53-12 + DO-7) Antibody

BNUM0723-50 50uL
EUR 395
Description: Primary antibody against p53(BP53-12 + DO-7), 1mg/mL

p53; Clone DO-7 (Ready-To-Use)

A00021-0002 2 ml
EUR 168

p53; Clone DO-7 (Ready-To-Use)

A00021-0007 7 ml
EUR 287

p53; Clone DO-7 (Ready-To-Use)

A00021-0025 25 ml
EUR 882

p53; Clone DO-1 (Ready-To-Use)

A00027-0007 7 ml
EUR 116

p53; Clone DO-1 (Ready-To-Use)

A00027-0025 25 ml
EUR 280

p53; Clone DO-1 (Ready-To-Use)

A20027-0002 2 ml
EUR 84

Major Histocompatibility Complex Class II DO alpha Protein

20-abx261103
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug

Major Histocompatibility Complex Class II DO beta Protein

20-abx261318
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug

p53 Cocktail; Clones Pab 1801 plus DO-7

A00093 6 ml
EUR 282

p53 Cocktail; Clones Pab 1801 plus DO-7

A00093.0025 25 ml
EUR 725

p53 Cocktail; Clones Pab 1801 plus DO-7

A20093 2 ml
EUR 165

p53 Tumor Suppressor Protein; Clone DO-7 (Concentrate)

RA0319-C.1 0.1 ml
EUR 125

p53 Tumor Suppressor Protein; Clone DO-7 (Concentrate)

RA0319-C.5 0.5 ml
EUR 300

8 channel ph/ec/sal/tds/res/do meter

C3062 ea
EUR 1620

Monoclonal p53 Tumor Suppressor Protein Antibody, Clone: DO-7

AMM01371G 7 ml
EUR 484
Description: A Monoclonal antibody against Human p53 Tumor Suppressor Protein. The antibodies are raised in Mouse and are from clone DO-7. This antibody is applicable in WB, IHC and IF, FC

Arabidopsis thaliana Putative protease Do-like 12, mitochondrial (DEGP12)

1-CSB-EP873288DOA
  • EUR 611.00
  • EUR 309.00
  • EUR 1827.00
  • EUR 939.00
  • EUR 1218.00
  • EUR 397.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Arabidopsis thaliana Putative protease Do-like 12, mitochondrial(DEGP12) expressed in E.coli

Major Histocompatibility Complex, Class II, DO Alpha (HLA-DOA) Antibody

20-abx133718
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul

Major Histocompatibility Complex, Class II, DO Beta (HLA-DOB) Antibody

20-abx125944
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Major Histocompatibility Complex, Class II, DO Beta (HLA-DOB) Antibody

20-abx125945
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

We have generated a system of two hybrid proteins containing parts of GAL4: the GAL4 DNA-binding domain fused to a protein ‘X’ and a GAL4 activating region fused to a protein ‘Y’. If X and Y can form a protein-protein complex and reconstitute proximity of the GAL4 domains, transcription of a gene regulated by UASG occurs.

We have tested this system using two yeast proteins that are known to interact–SNF1 and SNF4. High transcriptional activity is obtained only when both hybrids are present in a cell. This system may be applicable as a general method to identify proteins that interact with a known protein by the use of a simple galactose selection.