High-yield preparation of isolated rat liver parenchymal cells: a biochemical and fine structural study.

A new technique employing continuous recirculating perfusion of the rat liver in situ, shaking of the liver in buffer in vitro, and filtration of the tissue through nylon mesh, results in the conversion of about 50% of the liver into intact, isolated parenchymal cells. The perfusion media consist of: (a) calcium-free Hanks’ solution containing 0.05% collagenase and 0.10% hyaluronidase, and (b) magnesium and calcium-free Hanks’ solution containing 2 mM ethylenediaminetetraacetate. Biochemical and morphologic studies indicate that the isolated cells are viable.

Human Mesenchymal Stem Cells - Bone Marrow Derived
MSC001	Neuromics	500,000+ Cells	EUR 1288

Human Mesenchymal Stem Cells-Green Fluorescent-Labeled
SC00A2	Neuromics	500000	EUR 962

Human Mesenchymal Stem Cells-Red Fluorescent-Labeled
SC00A3	Neuromics	500000	EUR 962

Human Mesenchymal Stem Cells Expressing Oct3/4
SC00A6	Neuromics	500000	EUR 1184

Immortalized Human Pancreatic Mesenchymal Stem Cells - SV40
T0167	ABM	1x106 cells / 1.0 ml	Ask for price

Immortalized Human Pancreatic Mesenchymal Stem Cells - hTERT
T0168	ABM	1x106 cells / 1.0 ml	Ask for price

Immortalized Human Pancreatic Mesenchymal Stem Cells - Myc
T0169	ABM	1x106 cells / 1.0 ml	Ask for price

Immortalized Human Pancreatic Mesenchymal Stem Cells - Ras
T0170	ABM	1x106 cells / 1.0 ml	Ask for price

Immortalized Dog Mesenchymal Stem Cells-HPV E6/E7
T0352	ABM	1x106 cells / 1.0 ml	Ask for price

Immortalized Human Bone Marrow Mesenchymal Stem Cells SV40
T0520	ABM	1x106 cells / 1.0 ml	Ask for price

MSCGro-Mesenchymal Stem Cell complete, LS
SC00B1-500	Neuromics	500 ml	EUR 304

MSCGro-Humanized Mesenchymal Stem Cell, complete
SC00B2-500	Neuromics	500 ml	EUR 375

MSCGro-Mesenchymal Stem Cell, complete, SF
SC00B3-500	Neuromics	500 ml	EUR 388

Immortalized Mouse Calvarial Mesenchymal Progenitor Cells (iCALs)
T0019	ABM	1x106 cells / 1.0 ml	Ask for price

Immortalized Human Bone Marrow Mesenchymal Cells - hTERT
T0523	ABM	1x106 cells / 1.0 ml	Ask for price

Immortalized mouse adipose-derived mesenchymal cells (iMADs)
T0541	ABM	1x106 cells / 1.0 ml	Ask for price

Immortalized Rat Metanephric Mesenchymal Precursor Cells (KMM)
T0622	ABM	1x106 cells / 1.0 ml	Ask for price

Neural Stem Cells (iPSC from Blood Cells; Male)
ASE-9234	Applied StemCell	1 vial (1 x 10^6)	EUR 414.5
Description: 12 month

Neural Stem Cells (iPSC from Blood Cells; Female)
ASE-9234F	Applied StemCell	1 vial (1 x 10^6)	EUR 414.5
Description: 12 month

MSCGro-Humanized Mesenchymal Stem Cell Clinical Grade Medium, SF
SC00B4-500	Neuromics	500 ml	EUR 362

Immortalized Mouse Floxed Fam20c Dental Papillar Mesenchymal Cells
T0039	ABM	1x106 cells / 1.0 ml	Ask for price

Immortalized Mouse Urogenital Sinus Mesenchymal Cells (UGSM-2)
T0653	ABM	1x106 cells / 1.0 ml	Ask for price

Immortalized Mouse Atrioventricular Cushion Mesenchymal Cells (tsA58-AVM)
T0670	ABM	1x106 cells / 1.0 ml	Ask for price

Immortalized Canine Bone Marrow Mesenchymal Stromal Cells (DS1)
T0687	ABM	1x106 cells / 1.0 ml	Ask for price

Human Neural Stem Cells Differentiation Kit
abx298022-100ml	Abbexa	100 ml	EUR 314

Human Neural Stem Cells Expansion Kit
abx298023-1Kit	Abbexa	1 Kit	EUR 272

Immortalized Human Bone Marrow Mesenchymal Stromal Cells - hTERT (iMSC3)
T0529	ABM	1x106 cells / 1.0 ml	Ask for price

Immortalized Mouse Subcutaneous Adipose Multipotent Mesenchymal Cells (m17.ASC)
T0542	ABM	1x106 cells / 1.0 ml	Ask for price

Human Bone Marrow CD34+ Stem Cells, Cryopreserved
BMCD34-C0.5M	101Bio	-	Ask for price

Human Bone Marrow CD34+ Stem Cells, Cryopreserved
BMCD34-C1M	101Bio	-	Ask for price

Human Bone Marrow CD34+ Stem Cells, Cryopreserved
BMCD34-C5M	101Bio	-	Ask for price

Human Bone Marrow CD34+ Stem Cells, Fresh
BMCD34-F0.5M	101Bio	-	Ask for price

Human Bone Marrow CD34+ Stem Cells, Fresh
BMCD34-F5M	101Bio	-	Ask for price

Immortalized Mouse Intestinal Crypt Stem Cells (iMICs)
T0564	ABM	1x106 cells / 1.0 ml	Ask for price

Immortalized Human Amniotic Fluid Stem Cells - SV40
T0903	ABM	1x106 cells / 1.0 ml	Ask for price

Immortalized Mouse Subcutaneous Adipose Multipotent Mesenchymal Cells (m17.ASC-GFP)
T0543	ABM	1x106 cells / 1.0 ml	Ask for price

ARE-GFP (Bsd) Lentivirus
LVP981-B	GenTarget	1x107 IFU/ml x 200ul	EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 4 tandem repeats of antioxidant response element (ARE). This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

ARE-GFP (Neo) Lentivirus
LVP981-N	GenTarget	1x107 IFU/ml x 200ul	EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 4 tandem repeats of antioxidant response element (ARE). This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

ARE-GFP (Puro) Lentivirus
LVP981-P	GenTarget	1x107 IFU/ml x 200ul	EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 4 tandem repeats of antioxidant response element (ARE). This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

ARE-GFP (RFP) Lentivirus
LVP981-R	GenTarget	1x107 IFU/ml x 200ul	EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 4 tandem repeats of antioxidant response element (ARE). This lentivirus also contain the RFP selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

ARE-RFP (Bsd) Lentivirus
LVP982-B	GenTarget	1x107 IFU/ml x 200ul	EUR 552
Description: Pre-made lentivirus express RFP reporter under a minimal promoter that embedded 4 tandem repeats of antioxidant response element (ARE). This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

ARE-RFP (GFP) Lentivirus
LVP982-G	GenTarget	1x107 IFU/ml x 200ul	EUR 552
Description: Pre-made lentivirus express RFP reporter under a minimal promoter that embedded 4 tandem repeats of antioxidant response element (ARE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

ARE-RFP (Neo) Lentivirus
LVP982-N	GenTarget	1x107 IFU/ml x 200ul	EUR 552
Description: Pre-made lentivirus express RFP reporter under a minimal promoter that embedded 4 tandem repeats of antioxidant response element (ARE). This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

ARE-RFP (Puro) Lentivirus
LVP982-P	GenTarget	1x107 IFU/ml x 200ul	EUR 552
Description: Pre-made lentivirus express RFP reporter under a minimal promoter that embedded 4 tandem repeats of antioxidant response element (ARE). This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

ARE-Luc (Bsd) Lentivirus
LVP983-B	GenTarget	1x107 IFU/ml x 200ul	EUR 552
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter that embedded 4 tandem repeats of antioxidant response element (ARE). This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

ARE-Luc (GFP) Lentivirus
LVP983-G	GenTarget	1x107 IFU/ml x 200ul	EUR 552
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter that embedded 4 tandem repeats of antioxidant response element (ARE). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

ARE-Luc (Neo) Lentivirus
LVP983-N	GenTarget	1x107 IFU/ml x 200ul	EUR 552
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter that embedded 4 tandem repeats of antioxidant response element (ARE). This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

ARE-Luc (Puro) Lentivirus
LVP983-P	GenTarget	1x107 IFU/ml x 200ul	EUR 552
Description: Pre-madelentivirus express Firefly luciferase reporter under a minimal promoter that embedded 4 tandem repeats of antioxidant response element (ARE). This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

ARE-Luc (RFP) Lentivirus
LVP983-R	GenTarget	1x107 IFU/ml x 200ul	EUR 552
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter that embedded 4 tandem repeats of antioxidant response element (ARE). This lentivirus also contain the RFP selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

ARE-Rluc (Bsd) Lentivirus
LVP984-B	GenTarget	1x107 IFU/ml x 200ul	EUR 552
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter that embedded 4 tandem repeats of antioxidant response element (ARE). This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

ARE-Rluc (GFP) Lentivirus
LVP984-G	GenTarget	1x107 IFU/ml x 200ul	EUR 552
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter that embedded 4 tandem repeats of antioxidant response element (ARE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

ARE-Rluc (Neo) Lentivirus
LVP984-N	GenTarget	1x107 IFU/ml x 200ul	EUR 552
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter that embedded 4 tandem repeats of antioxidant response element (ARE). This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

ARE-Rluc (Puro) Lentivirus
LVP984-P	GenTarget	1x107 IFU/ml x 200ul	EUR 552
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter that embedded 4 tandem repeats of antioxidant response element (ARE). This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

ARE-Rluc (RFP) Lentivirus
LVP984-R	GenTarget	1x107 IFU/ml x 200ul	EUR 552
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter that embedded 4 tandem repeats of antioxidant response element (ARE). This lentivirus also contain the RFP selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

pGL4.37[luc2P ARE Hygro]
PVT17453	Lifescience Market	2 ug	EUR 300

Mesenchymal Cell Kit
abx290006-50test	Abbexa	50 test	EUR 2458

Immortalized Mouse Conditionally Reprogrammed Otic Stem Cells (CR-OSC)
T0372	ABM	1x106 cells / 1.0 ml	Ask for price

Immortalized Human Neural Stem Cells - L-Myc (LM-NSC008)
T0682	ABM	1x106 cells / 1.0 ml	EUR 688

ARE-GFP (Puro) Lentivirusin PBS
LVP981-P-PBS	GenTarget	1x108 IFU/ml x 200ul	EUR 710
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 4 tandem repeats of antioxidant response element (ARE). This lentivirus also contain the puromycin selection marker under RSV promoter. Lentivirus concentrated and provided in PBS solution.

PCS-500-011 Human pre-adipose derived mesenchymal stem cell: >1x10^10 frozen exosomes
EXOP-140A-1	SBI	50 ug	EUR 587

PCS-500-012 Human bone marrow-derived mesenchymal stem cell line: >1x10^10 frozen exosomes
EXOP-145A-1	SBI	50 ug	EUR 587

ARE-GFP (Bsd) Lentivirus in PBS
LVP981-B-PBS	GenTarget	1x108 IFU/ml x 200ul	EUR 710
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 4 tandem repeats of antioxidant response element (ARE). This lentivirus also contain the Blasticidin selection marker under RSV promoter. Lentivirus concentrated and provided in PBS solution.

ARE-GFP (Neo) Lentivirus in PBS
LVP981-N-PBS	GenTarget	1x108 IFU/ml x 200ul	EUR 710
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 4 tandem repeats of antioxidant response element (ARE). This lentivirus also contain the Neomycin selection marker under RSV promoter. Lentivirus concentrated and provided in PBS solution.

ARE-GFP (RFP) Lentivirus in PBS
LVP981-R-PBS	GenTarget	1x108 IFU/ml x 200ul	EUR 710
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 4 tandem repeats of antioxidant response element (ARE). This lentivirus also contain the RFP selection marker under the constitutive RSV promoter. Lentivirus concentrated and provided in PBS solution.

ARE-RFP (Bsd) Lentivirus in PBS
LVP982-B-PBS	GenTarget	1x108 IFU/ml x 200ul	EUR 710
Description: Pre-made lentivirus express RFP reporter under a minimal promoter that embedded 4 tandem repeats of antioxidant response element (ARE). This lentivirus also contain the Blasticidin selection marker under RSV promoter. Lentivirus concentrated and provided in PBS solution.

ARE-RFP (GFP) Lentivirus in PBS
LVP982-G-PBS	GenTarget	1x108 IFU/ml x 200ul	EUR 710
Description: Pre-made lentivirus express RFP reporter under a minimal promoter that embedded 4 tandem repeats of antioxidant response element (ARE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter. Lentivirus concentrated and provided in PBS solution.

ARE-RFP (Neo) Lentivirus in PBS
LVP982-N-PBS	GenTarget	1x108 IFU/ml x 200ul	EUR 710
Description: Pre-made lentivirus express RFP reporter under a minimal promoter that embedded 4 tandem repeats of antioxidant response element (ARE). This lentivirus also contain the Neomycin selection marker under RSV promoter. Lentivirus concentrated and provided in PBS solution.

ARE-RFP (Puro) Lentivirus in PBS
LVP982-P-PBS	GenTarget	1x108 IFU/ml x 200ul	EUR 710
Description: Pre-made lentivirus express RFP reporter under a minimal promoter that embedded 4 tandem repeats of antioxidant response element (ARE). This lentivirus also contain the puromycin selection marker under RSV promoter. Lentivirus concentrated and provided in PBS solution.

ARE-Luc (Bsd) Lentivirus in PBS
LVP983-B-PBS	GenTarget	1x108 IFU/ml x 200ul	EUR 710
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter that embedded 4 tandem repeats of antioxidant response element (ARE). This lentivirus also contain the Blasticidin selection marker under RSV promoter. Lentivirus concentrated and provided in PBS solution.

ARE-Luc (GFP) Lentivirus in PBS
LVP983-G-PBS	GenTarget	1x108 IFU/ml x 200ul	EUR 710
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter that embedded 4 tandem repeats of antioxidant response element (ARE). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter. Lentivirus concentrated and provided in PBS solution.

ARE-Luc (Neo) Lentivirus in PBS
LVP983-N-PBS	GenTarget	1x108 IFU/ml x 200ul	EUR 710
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter that embedded 4 tandem repeats of antioxidant response element (ARE). This lentivirus also contain the Neomycin selection marker under RSV promoter. Lentivirus concentrated and provided in PBS solution.

ARE-Luc (Puro) Lentivirus in PBS
LVP983-P-PBS	GenTarget	1x108 IFU/ml x 200ul	EUR 710
Description: Pre-madelentivirus express Firefly luciferase reporter under a minimal promoter that embedded 4 tandem repeats of antioxidant response element (ARE). This lentivirus also contain the puromycin selection marker under RSV promoter. Lentivirus concentrated and provided in PBS solution.

ARE-Luc (RFP) Lentivirus in PBS
LVP983-R-PBS	GenTarget	1x108 IFU/ml x 200ul	EUR 710
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter that embedded 4 tandem repeats of antioxidant response element (ARE). This lentivirus also contain the RFP selection marker under the constitutive RSV promoter. Lentivirus concentrated and provided in PBS solution.

ARE-Rluc (Bsd) Lentivirus in PBS
LVP984-B-PBS	GenTarget	1x108 IFU/ml x 200ul	EUR 710
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter that embedded 4 tandem repeats of antioxidant response element (ARE). This lentivirus also contain the Blasticidin selection marker under RSV promoter. Lentivirus concentrated and provided in PBS solution.

ARE-Rluc (GFP) Lentivirus in PBS
LVP984-G-PBS	GenTarget	1x108 IFU/ml x 200ul	EUR 710
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter that embedded 4 tandem repeats of antioxidant response element (ARE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter. Lentivirus concentrated and provided in PBS solution.

ARE-Rluc (Neo) Lentivirus in PBS
LVP984-N-PBS	GenTarget	1x108 IFU/ml x 200ul	EUR 710
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter that embedded 4 tandem repeats of antioxidant response element (ARE). This lentivirus also contain the Neomycin selection marker under RSV promoter. Lentivirus concentrated and provided in PBS solution.

ARE-Rluc (Puro) Lentivirus in PBS
LVP984-P-PBS	GenTarget	1x108 IFU/ml x 200ul	EUR 710
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter that embedded 4 tandem repeats of antioxidant response element (ARE). This lentivirus also contain the puromycin selection marker under RSV promoter. Lentivirus concentrated and provided in PBS solution.

ARE-Rluc (RFP) Lentivirus in PBS
LVP984-R-PBS	GenTarget	1x108 IFU/ml x 200ul	EUR 710
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter that embedded 4 tandem repeats of antioxidant response element (ARE). This lentivirus also contain the RFP selection marker under the constitutive RSV promoter. Lentivirus concentrated and provided in PBS solution.

pGL4.19-TA-ARE response element Plasmid
PVTB00984-2a	Lifescience Market	2 ug	EUR 356

Human Uncharacterized hematopoietic stem/progenitor cells protein MDS032 / MDS032 (USE1) ELISA Kit
abx384157-96tests	Abbexa	96 tests	EUR 911

Vimentin (Mesenchymal Cell Marker) (VM452) Antibody
BNUB0452-50	Biotium	50uL	EUR 405
Description: Primary antibody against Vimentin (Mesenchymal Cell Marker) (VM452), 1mg/mL

Human Epithelial Mesenchymal Transition Primer Library
HEMT-I	Real Time Primers	1 set	EUR 548

Mouse Epithelial-Mesenchymal Transition Primer Library
MEMT-I	Real Time Primers	1 set	EUR 548

Stem Cell Kit
20-abx290004	Abbexa	EUR 1024.00 EUR 1288.00	25 tests 50 tests

STEM CELL KIT
SCK(V25)	ImmunoStep	25 test	EUR 830

STEM CELL KIT
SCK(V50)	ImmunoStep	50 test	EUR 1083.5

Dendritic Cells/B Cells Antibody
abx412775-02mg	Abbexa	0.2 mg	EUR 565

anti-CD45 RA B-cells, T-cells, NK-cells
516-A-01mg	Virogen	0,1 mg	EUR 267.5
Description: anti-CD45 RA B-cells, T-cells, NK-cells

anti-CD45 RA B-cells, T-cells, NK-cells
516-A-1000ug	Virogen	1000 ug	EUR 1282.5
Description: anti-CD45 RA B-cells, T-cells, NK-cells

Vimentin (Mesenchymal Cell Marker); Clone VM452 (Concentrate)
RA0337-C.1	ScyTek Laboratories	0.1 ml	EUR 125

Vimentin (Mesenchymal Cell Marker); Clone VM452 (Concentrate)
RA0337-C.5	ScyTek Laboratories	0.5 ml	EUR 300

Vimentin (Mesenchymal Cell Marker); Clone LN6 (Concentrate)
RA0338-C.1	ScyTek Laboratories	0.1 ml	EUR 125

Vimentin (Mesenchymal Cell Marker); Clone LN6 (Concentrate)
RA0338-C.5	ScyTek Laboratories	0.5 ml	EUR 247

C127 cells
S0006001	Addexbio	One Frozen vial	EUR 485

HASM cells
P0001001	Addexbio	One Frozen vial	EUR 777

HBSM cells
P0007001	Addexbio	One Frozen vial	EUR 777

They respire in a medium containing calcium ions, synthesize glucose from lactate, are impermeable to inulin, do not stain with trypan blue, and retain their structural integrity. Electron microscopy of biopsies taken during and after perfusion reveals that desmosomes are quickly cleaved. Hemidesmosome-containing areas of the cell membrane invaginate and appear to pinch off and migrate centrally.

Tight and gap junctions, however, persist on the intact, isolated cells, retaining small segments of cytoplasm from formerly apposing parenchymal cells.