March 19, 2024
Zdolność bakterii morskiej Pseudomonas fluorescens BA3SM1 do przeciwdziałania toksyczności nanocząsteczek CdSe.

Zdolność bakterii morskiej Pseudomonas fluorescens BA3SM1 do przeciwdziałania toksyczności nanocząsteczek CdSe.

W środowisku morskim bakterie z osadów przyujściowych i przybrzeżnych są jednymi z pierwszych celów zanieczyszczenia nanocząsteczkami; dlatego istotne jest poszerzenie wiedzy na temat interakcji między bakteriami a nanocząsteczkami. W pracy oceniono odpowiedź bakterii morskiej Pseudomonas fluorescens BA3SM1 na nanokryształy CdSe (CdSe NPs) o średnicy 3 nm (NP3) i 8 nm (NP8) za pomocą metod mikroskopowych, fizjologicznych, biochemicznych i proteomicznych. Obrazy z transmisyjnej mikroskopii elektronowej wykazały, że NP3 były w stanie penetrować bakterie, podczas gdy NP8 były silnie skoncentrowane wokół komórek, osadzone w dużych egzopolisacharydach.

W naszych warunkach eksperymentalnych oba rozmiary CdSe NP wywoływały spadek oddychania podczas stacjonarnej fazy wzrostu, podczas gdy tylko NP8 powodował opóźnienie wzrostu i zmniejszenie produkcji pyowerdyny. Analizy proteomiczne wykazały, że szczep zareagował na toksyczność CdSe NP, wywołując różne mechanizmy obronne, takie jak agregacja komórek, sekwestracja zewnątrzkomórkowa CdSe NP, skuteczna ochrona przed stresem oksydacyjnym, modyfikacje organizacji i właściwości otoczki oraz eksport kadmu. Ponadto BA3SM1 wykazywał zdolność biosorpcji 1,6 x 10 (16) NP3 / g suchej masy i 1,7 x 10 (15) NP8 / g suchej masy.

Zgodnie z naszą najlepszą wiedzą jest to pierwszy raport skupiający się na wpływie koloidalnych nanokryształów CdSe (CdSe NP) na morski szczep Pseudomonas fluorescens. CdSe NP są szeroko stosowane w przemyśle energii odnawialnej i niestety oczekuje się, że te zanieczyszczenia wkrótce pojawią się w środowisku morskim poprzez spływy powierzchniowe, ścieki miejskie i rzeki. Dlatego szczep ten wydaje się być obiecującym czynnikiem w procesach bioremediacji NP. Dane proteomiczne są dostępne za pośrednictwem ProteomeXchange z identyfikatorem PXD004012.

Zwiększenie tempa metabolizmu pomimo spadku masy ciała u dorosłej osy parazytoidalnej.

Tempo metabolizmu jest pozytywną funkcją masy ciała, regułą obowiązującą dla większości organizmów i podstawą kilku teorii ekologii metabolicznej. Jednak w przypadku owadów dorosłych różnorodność zależności między masą ciała a oddychaniem pozostaje niewyjaśniona. Celem pracy jest powiązanie metabolizmu oddechowego pasożyta z masą ciała i aktywnością żerowania. Porównaliśmy tempo metabolizmu grup samic pasożyta Eupelmus vuilleti, które głodują i żywią się żywicielem, zarejestrowane za pomocą respirometrii przez 7 dni, co odpowiada średniemu okresowi życia głodujących samic i ponad połowie życia żerujących samic. Dynamikę węglowodanów, lipidów i białek w organizmie żerujących samic określono ilościowo za pomocą technik biochemicznych.

Masa ciała i wszystkie składniki odżywcze ciała gwałtownie spadły od pierwszego dnia. Z kolei wytwarzany CO2 i zużyty O2 stale wzrastały. Głodujące samice wykazywały odwrotną tendencję, identyfikując żerowanie jako przyczynę przyspieszenia oddychania samic. Dwa uzupełniające się procesy fizjologiczne wyjaśniają nieoczekiwany związek między zwiększeniem tempa metabolizmu a spadkiem masy ciała. Po pierwsze, hemolimfa gospodarza jest wysoce niezrównoważonym pożywieniem, a nadmiar składników odżywczych (białka i węglowodany) należy usunąć, częściowo przez wydalanie, a częściowo przez oddychanie.

Po drugie, żerująca młoda samica produkuje jaja w coraz większym tempie w pierwszej połowie swojego życia, co również zwiększa oddychanie. Zakładamy, że zmienny w czasie udział tempa metabolizmu w procesach karmienia i reprodukcji uzupełnia udział masy strukturalnej i prowadzi do obserwowanego trendu. Rozszerzamy nasze wyjaśnienia na inne grupy owadów i omawiamy potencjał zjednoczenia przy użyciu teorii dynamicznego budżetu energetycznego.

Przypuszczalnie zidentyfikowane rodzaje obejmują Arthrobacter, Brevibacterium, Bacillus, Cornyebacterium, Actinomyces, Aureobacterium, Chromobacterium i Mycobacterium. Pseudomonas spp. nie zostały odzyskane. Pięćdziesiąt procent zgrupowanych operacyjnych jednostek taksonomicznych (OTU) nie zostało zidentyfikowanych. Trzydzieści procent skupionych OTU stanowiły nieregularne, asporogenne pałeczki Gram-dodatnie. Zbiorowiska bakteryjne różniły się w zależności od miejsca, a pożywka izolacyjna miała silny wpływ na odzyskane szczepy.

Zdolność bakterii morskiej Pseudomonas fluorescens BA3SM1 do przeciwdziałania toksyczności nanocząsteczek CdSe.

Multiscale Vibrational Spectroscopic Approach for Identification and Biochemical Characterisation of Pollen.

Analiza ziaren pyłku dostarcza cennych informacji na temat biologii, ekologii, medycyny sądowej, zmian klimatycznych, migracji owadów, źródeł pożywienia i alergenów lotnych. Spektroskopie wibracyjne (w podczerwieni i Ramana) oferują chemiczną charakterystykę pyłku poprzez identyfikowalne cechy spektralne bez jakiejkolwiek wstępnej obróbki próbki. Porównaliśmy poziom informacji chemicznych, które można uzyskać za pomocą różnych wieloskalowych technik spektroskopii oscylacyjnej.

Pyłki z 15 różnych gatunków Pinales (drzew iglastych) mierzono siedmioma metodami podczerwieni i ramanowskiej. W celu uzyskania widm w podczerwieni wykonano pomiary zarówno odbicia, jak i transmisji na zmielonych i nienaruszonych ziarnach pyłku (pomiary masowe), dodatkowo widma w podczerwieni uzyskano metodą mikrospektroskopii pomiarów wieloziarnistych i pojedynczych ziaren pyłku. W przypadku pomiarów mikrospektroskopii ramanowskiej widma uzyskano z tych samych ziaren pyłku poprzez ogniskowanie dwóch różnych podstruktur ziaren pyłku. Dane widmowe z siedmiu metodologii zostały zintegrowane w jeden model danych za pomocą analizy Consensus Principal Component Analysis w celu uzyskania relacji między sygnaturami molekularnymi śledzonymi różnymi technikami.

Spektroskopia oscylacyjna umożliwiła charakterystykę biochemiczną pyłku i wykrycie zmienności filogenetycznej. Różnice widmowe były wyraźnie związane z określonymi składnikami chemicznymi, takimi jak lipidy, węglowodany, karotenoidy i sporopolleniny. Rozległe różnice między pyłkiem Cedrus a resztą rodziny Pinaceae były jednoznacznie związane ze składem molekularnym sporopollenin w ścianie ziarna pyłku, podczas gdy w pyłku Picea występuje widocznie wyższe stężenie karotenoidów niż reszta rodziny.

Chemically Modified siRNA

M1254-9 each
EUR 686.4

Chemically Modified siRNA Set C

M1270-1 each
EUR 1024.8

Chemically Modified siRNA Set D

M1271-1 each
EUR 1208.4

Chemically Competent Cell

20-abx098066
  • EUR 510.00
  • EUR 543.60
  • 1 ml
  • 2 ml

Ar.A4 Chemically Competent Cell

ACC-116 10 tubes, 20 tubes, 50 tubes, 100 tubes Ask for price
Description: Applications: Ar.A4 Chemically Competent Cell is suitable for transgenic operations of corn, tobacco, carrot, licorice and other plants.

DMT Chemically Competent Cell

20-abx098071
  • EUR 526.80
  • EUR 493.20
  • 1 ml
  • 500 ul

DB3.1 Chemically Competent Cell

abx098865-1ml 1 ml
EUR 543.6

Chemically Competent Cell (StrR)

20-abx098064
  • EUR 493.20
  • EUR 526.80
  • 1 ml
  • 2 ml

BL21 Chemically Competent Cell

20-abx098105
  • EUR 526.80
  • EUR 560.40
  • EUR 493.20
  • 1 ml
  • 2 ml
  • 500 ul

AGL1 Chemically Competent Cell

ACC-105 10 tubes, 20 tubes, 50 tubes, 100 tubes Ask for price
Description: Applications: AGL1 Chemically Competent Cell is suitable for transgenic operations of rice, Arabidopsis, poplar and other plants.

Ar.Qual Chemically Competent Cell

ACC-117 10 tubes, 20 tubes, 50 tubes, 100 tubes Ask for price
Description: Applications: Ar.Qual Chemically Competent Cell is suitable for transgenic operations of corn, tobacco, tomato, citrus and other plants.

K599 Chemically Competent Cell

ACC-121 10 tubes, 20 tubes, 50 tubes, 100 tubes Ask for price
Description: Applications: K599 Chemically Competent Cell is suitable for transgenic operations of cucurbitaceae, leguminosae, solanaceae and other plants.

C58C1 Chemically Competent Cell

ACC-119 10 tubes, 20 tubes, 50 tubes, 100 tubes Ask for price
Description: Applications: C58C1 Chemically Competent Cell is suitable for transgenic operations of rosaceae, apocynaceae, leguminosae, solanaceae, astragalus, tobacco and other plants.

TOP10 Chemically Competent Cell

ACC-129 10 tubes, 20 tubes, 50 tubes, 100 tubes Ask for price

GV3101 Chemically Competent Cell

ACC-100 10 tubes, 20 tubes, 50 tubes, 100 tubes Ask for price
Description: Applications: GV3101 Chemically Competent Cell is suitable for transgenic operations of Arabidopsis, tobacco, corn, potatoes and other plants.

EHA105 Chemically Competent Cell

ACC-103 10 tubes, 20 tubes, 50 tubes, 100 tubes Ask for price
Description: Applications: EHA105 Chemically Competent Cell is suitable for transgenic operations of rice, tobacco and other plants.

EHA101 Chemically Competent Cell

ACC-108 10 tubes, 20 tubes, 50 tubes, 100 tubes Ask for price
Description: Applications: EHA101 Chemically Competent Cell is suitable for transgenic operations of corn, rice, tobacco and other plants.

MSU440 Chemically Competent Cell

ACC-118 10 tubes, 20 tubes, 50 tubes, 100 tubes Ask for price
Description: Applications: MSU440 Chemically Competent Cell is suitable for transgenic operations of corn, tobacco, tea tree, artemisia annua and other plants.

Ar.1193 Chemically Competent Cell

ACC-120 10 tubes, 20 tubes, 50 tubes, 100 tubes Ask for price
Description: Applications: Ar.1193 Chemically Competent Cell is suitable for transgenic operations of leguminosae, solanaceae and other plants.

LBA4404 Chemically Competent Cell

ACC-107 10 tubes, 20 tubes, 50 tubes, 100 tubes Ask for price
Description: Applications: LBA4404 Chemically Competent Cell is suitable for transgenic operations of tomatoes, tobacco and other plants.

T1 Phage Chemically Comptent Cell

20-abx098070
  • EUR 510.00
  • EUR 560.40
  • EUR 484.80
  • 1 ml
  • 2 ml
  • 500 ul

Chemically Defined Lipid Concentrate

EN-CELL-010 5ml
EUR 30

5 alpha Chemically Competent Cell

20-abx098065
  • EUR 493.20
  • EUR 526.80
  • 1 ml
  • 2 ml

BL21 (DE3) Chemically Competent Cell

20-abx098101
  • EUR 526.80
  • EUR 560.40
  • EUR 493.20
  • 1 ml
  • 2 ml
  • 500 ul

DE3 Chemically Competent Cell (CamR)

20-abx098103
  • EUR 526.80
  • EUR 577.20
  • EUR 493.20
  • 1 ml
  • 2 ml
  • 500 ul

Blue Chemically Competent Cell (TetR)

20-abx098068
  • EUR 510.00
  • EUR 543.60
  • 1 ml
  • 2 ml

AGL1 (pSoup) Chemically Competent Cell

ACC-106 10 tubes, 20 tubes, 50 tubes, 100 tubes Ask for price
Description: Applications: AGL1 (pSoup) Chemically Competent Cell is suitable for transgenic operations of Arabidopsis, tobacco, corn, potatoes and other plants.

*Human sc-tPA Chemically Inactivated

IHUSCTPACMIN100UG each
EUR 443
Description: *Human sc-tPA Chemically Inactivated

*Human sc-tPA Chemically Inactivated

IHUSCTPACMIN1MG each
EUR 3684
Description: *Human sc-tPA Chemically Inactivated

GV3101 (pSoup) Chemically Competent Cell

ACC-101 10 tubes, 20 tubes, 50 tubes, 100 tubes Ask for price
Description: Applications: GV3101 (pSoup) Chemically Competent Cell is suitable for transgenic operations of Arabidopsis, tobacco, corn, potatoes and other plants.

EHA105 (pSoup) Chemically Competent Cell

ACC-104 10 tubes, 20 tubes, 50 tubes, 100 tubes Ask for price
Description: Applications: EHA105 (pSoup) Chemically Competent Cell is suitable for transgenic operations of rice, tobacco and other plants.

DE3 Chemically Comptent Cell (KanR, TetR)

20-abx098104
  • EUR 526.80
  • EUR 493.20
  • 1 ml
  • 500 ul

JM109 Chemically Competent Cells - 6x200µl

1062-24 200μL
EUR 149
Description: Intact Genomics JM109 chemically competent E. coli cells are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning.

HB101 Chemically Competent Cells - 6x100µl

1071-12 100μL
EUR 99
Description: Intact Genomics (ig®) HB101 chemically competent E. coli cells are suitable for high-efficiency transformation in a wide variety of applications such as cloning and sub-cloning. E. coli HB101 is a K12 x B hybrid strain, containing the recA13 mutation that minimizes recombination and helps insert stability. In addition, it carries the hsdS20(rB-mB-) restriction minus genotype which prevents cleavage of cloned DNA by endogenous restriction enzymes. HB101 strain does not support Alpha-complementation for blue/white screening

JM109 Chemically Competent Cells - 12x200µl

1062-48 200μL
EUR 255
Description: Intact Genomics JM109 chemically competent E. coli cells are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning.

HB101 Chemically Competent Cells - 24x100µl

1071-24 100μL
EUR 315
Description: Intact Genomics (ig®) HB101 chemically competent E. coli cells are suitable for high-efficiency transformation in a wide variety of applications such as cloning and sub-cloning. E. coli HB101 is a K12 x B hybrid strain, containing the recA13 mutation that minimizes recombination and helps insert stability. In addition, it carries the hsdS20(rB-mB-) restriction minus genotype which prevents cleavage of cloned DNA by endogenous restriction enzymes. HB101 strain does not support Alpha-complementation for blue/white screening

MG1655 Chemically Competent Cells - 12x50µl

1076-12 50μL
EUR 190
Description: Intact Genomics (ig®) chemically competent MG1655 chemically competent cells are suitable for routine DNA transformation and other research purposes. MG1655 is the “wild type” K-12 strain with minimal genetic manipulation having only been cured of the temperate bacteriophage lambda and F plasmid by ultraviolet light and acridine orange

Blue Chemically Competent Cell (TetR, CamR)

20-abx098069
  • EUR 526.80
  • EUR 577.20
  • 1 ml
  • 2 ml

BL21 (DE3) pLysS Chemically Competent Cell

20-abx098102
  • EUR 526.80
  • EUR 560.40
  • EUR 493.20
  • 1 ml
  • 2 ml
  • 500 ul

BL21(DE3) Chemically Competent Cells - 12x50µl

1051-12 12/PK
EUR 204

BL21(DE3) Chemically Competent Cells - 24x50µl

1051-24 24/PK
EUR 297.6

BL21(DE3) Chemically Competent Cells - 6x200µl

1054-24 6/PK
EUR 235.2

DL39 (DE3) Chemically Competent Cells - 12x50µl

1061-12 12/PK
EUR 382.8

DL39 (DE3) Chemically Competent Cells - 24x50µl

1061-24 24/PK
EUR 621.6

DL39 (DE3) Chemically Competent Cells - 6x200µl

1064-24 200μL
EUR 465
Description: Intact Genomics chemically competent DL39 (DE3) E. coli cells are specific for transformation and protein expression in order to uniformly and specifically label :e.g. phenylalanine or leucine residues. DL39 (DE3) can also be used to reduce NMR cross-labeling via transaminase activity for valine, leucine, isoleucine, aspartate, phenylalanine, tyrosine and tryptophan residuesProduct Includes:DL39 (DE3) competent cellspUC19 control DNARecovery medium

Chemically Competent Agrobacterium Combo Pack

1091-12 50μL
EUR 945
Description: Intact Genomics Chemically Competent Agrobacterium Combo Pack is perfect for scientists who require multiple agrobacterium competent cell strains for their research. The ElectroCompetent Agrobacterium Combo Pack contains 3 50µl aliquots of GV3101, AGL1, EHA105 and LBA4404 along with pCambia and our proprietary Agro Recovery Media.The GV3101 strain has a C58 chromosomal background with rifampicin resistance and the Ti plasmid pMP90 (pTiC58DT-DNA) with gentamicin resistance. The GV3101 Ti plasmid has the T-DNA region sequences deleted and transformation with a binary vector containing the missing T-region results in a functional T-DNA binary system that allows for transfer of genetic material into a host plant’s genome. Therefore, this system is often used for Agrobacterium-mediated transformation of several dicots such as Arabidopsis thaliana, tobacco, potato, and monocots like corn.The AGL1 strain has a C58 chromosomal background that carries an insertion mutation in its recA recombination gene which stabilizes recombinant plasmids. It also carries rifampicin and carbenicilin resistance in its genome for selection. AGL1 contains the Ti plasmid pTiBO542 from which the T-DNA region sequences have been deleted. Transformation with a binary vector containing the missing T-region results in a functional T-DNA binary system that allows for transfer of genetic material into a host plant’s genome. Therefore, this system is often used for Agrobacterium-mediated transformation of Arabidopsis thaliana as well as maize and other monocots.The LBA4404 strain is useful for transgenic operations of tomatoes, tobacco and other plants. LBA4404 contains a rifampicin resistance gene (rif). LBA4404 strain also contains a octoprine-type Ti plasmid pAL4404 without self-transport function, which contains the vir gene.The EHA105 strain is useful for transgenic operations of rice, tobacco and other plants. EHA105 contains a rifampicin resistance gene (rif). EHA105 strain also contains an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA) without self-transport function, which contains the vir gene.

Chemically Competent Cell (106 cfu / mu g DNA)

20-abx098067
  • EUR 526.80
  • EUR 493.20
  • 1 ml
  • 500 ul

BL21(DE3) Chemically Competent Cells - 12x200µl

1054-48 12/PK
EUR 384

JM109(DE3) Chemically Competent Cells - 6x200µl

1063-24 200μL
EUR 149
Description: Intact Genomics JM109(DE3) chemically competent E. coli cells are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. JM109(DE3) is useful for the high-level expression of genes cloned into vectors for expression of sequence downstream from the T7 promoter.

GV3101 (pSoup-p19) Chemically Competent Cell

ACC-102 10 tubes, 20 tubes, 50 tubes, 100 tubes Ask for price
Description: Applications: GV3101 (pSoup-p19) Chemically Competent Cell is suitable for transgenic operations of Arabidopsis, tobacco, corn, potatoes and other plants.

AGL1 Chemically Competent Agrobacterium - 6x50µl

1083-06 50μL
EUR 355
Description: The AGL1 strain (A. tumefaciens) has a C58 chromosomal background that carries an insertion mutation in its recA recombination gene which stabilizes recombinant plasmids. It also carries rifampicin and carbenicilin resistance in its genome for selection. AGL1 contains the Ti plasmid pTiBO542 from which the T-DNA region sequences have been deleted. Transformation with a binary vector containing the missing T-region results in a functional T-DNA binary system that allows for transfer of genetic material into a host plant’s genome. Therefore, this system is often used for Agrobacterium-mediated transformation of Arabidopsis thaliana as well as maize and other monocots.Product Includes:• AGL1 Chemically Competent cells• pCAMBIA control DNA• Recovery medium

JM109(DE3) Chemically Competent Cells - 12x200µl

1063-48 200μL
EUR 255
Description: Intact Genomics JM109(DE3) chemically competent E. coli cells are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. JM109(DE3) is useful for the high-level expression of genes cloned into vectors for expression of sequence downstream from the T7 promoter.

AGL1 Chemically Competent Agrobacterium - 18x50µl

1083-18 50μL
EUR 755
Description: The AGL1 strain (A. tumefaciens) has a C58 chromosomal background that carries an insertion mutation in its recA recombination gene which stabilizes recombinant plasmids. It also carries rifampicin and carbenicilin resistance in its genome for selection. AGL1 contains the Ti plasmid pTiBO542 from which the T-DNA region sequences have been deleted. Transformation with a binary vector containing the missing T-region results in a functional T-DNA binary system that allows for transfer of genetic material into a host plant’s genome. Therefore, this system is often used for Agrobacterium-mediated transformation of Arabidopsis thaliana as well as maize and other monocots.Product Includes:• AGL1 Chemically Competent cells• pCAMBIA control DNA• Recovery medium

C58C1 Chemically Competent Agrobacterium - 6x50µl

1086-06 50μL
EUR 355
Description: C58C1 Chemically Competent Agrobacterium cells are optimized for the highest transformation efficiency and are useful for various applications. The chromosomal background of C58C1 is C58. C58 is cured of the Ti plasmid pTiC58 resulting in C58C1. C58C1 Competent cells may be useful for transgenic operations that involve Arabidopsis and other plants. This Agrobacterium strain is streptomycin and rifampicin resistant.Reagents Included:• C58C1 Chemically Competent Agrobacterium• DNA (pCAMBIA1391z, 500 pg/µl)• Recovery medium

DH10B Chemically Competent Cells (ig10B) - 6x100µl

1012-12 6/PK
EUR 196.8

DH10B Chemically Competent Cells (ig10B) - 6x200µl

1014-24 6/PK
EUR 259.2

DH10B Chemically Competent Cells (ig10B) - 12x50µl

1011-12 12/PK
EUR 210

DH10B Chemically Competent Cells (ig10B) - 24x50µl

1011-24 24/PK
EUR 309.6

MSU440 Chemically Competent Agrobacterium- 6x50µl

1077-12 50μL
EUR 355
Description: Intact Genomics (ig®) MSU440 Chemically Competent Agrobacterium cells are made from a specific strain of Rhizobium rhizogenes (formerly Agrobacterium rhizogenes), Agrobacterium rhizogenes(str R) MSU440 Ri (agropine type). Agrobacterium rhizogenes is a soil-borne gram-negative bacterium that can infect most dicotyledons, a few monocotyledons and some gymnosperms. MSU440 Chemically Competent Agrobacterium are optimized for the highest transformation efficiencies and are useful for transgenic operations of corn, tobacco, wormwood, tea tree and other plants. MSU440 Agrobacterium rhizogenes strain contains agrobacterium-type Ri plasmid and displays streptomycin resistance.

GV3101 Chemically Competent Agrobacterium - 6x50µl

1082-06 50μL
EUR 355
Description: GV3101 strain (A. tumefaciens) has a C58 chromosomal background with rifampicin resistance and the Ti plasmid pMP90 (pTiC58DT-DNA) with gentamicin resistance. The GV3101 Ti plasmid has the T-DNA region sequences deleted and transformation with a binary vector containing the missing T-region results in a functional T-DNA binary system that allows for transfer of genetic material into a host plant’s genome. Therefore, this system is often used for Agrobacterium-mediated transformation of several dicots such as Arabidopsis thaliana, tobacco, potato, and monocots like corn.Product Includes:• GV3101 Chemically cells• pCAMBIA control DNA• Recovery medium

EHA105 Chemically Competent Agrobacterium - 6x50µl

1084-06 50μL
EUR 355
Description: Intact Genomics EHA105 Chemically Competent Agrobacterium cells are optimized for the highest transformation efficiencies which is ideal for applications requiring high transformation efficiencies, such as with cDNA or gDNA library construction. The EHA105 strain is useful for transgenic operations of rice, tobacco and other plants. EHA105 contains a rifampicin resistance gene (rif). EHA105 strain also contains an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA) without self-transport function, which contains the vir gene.Product Includes:• EHA105 Chemically Competent cells• pCAMBIA control DNA• Recovery medium

C58C1 Chemically Competent Agrobacterium - 18x50µl

1086-18 50μL
EUR 755
Description: C58C1 Chemically Competent Agrobacterium cells are optimized for the highest transformation efficiency and are useful for various applications. The chromosomal background of C58C1 is C58. C58 is cured of the Ti plasmid pTiC58 resulting in C58C1. C58C1 Competent cells may be useful for transgenic operations that involve Arabidopsis and other plants. This Agrobacterium strain is streptomycin and rifampicin resistant.Reagents Included:• C58C1 Chemically Competent Agrobacterium• DNA (pCAMBIA1391z, 500 pg/µl)• Recovery medium

DH10B Chemically Competent Cells (ig10B) - 12x100µl

1012-24 12/PK
EUR 297.6

DH10B Chemically Competent Cells (ig10B) - 24x100µl

1012-48 24/PK
EUR 459.6

DH10B Chemically Competent Cells (ig10B) - 12x200µl

1014-48 12/PK
EUR 434.4

Ar. A4 Chemically Competent Agrobacterium- 6x50µl

1072-06 50μL
EUR 355
Description: Intact Genomics (ig®) Ar.A4 Chemically Competent Agrobacterium cells are made from a specific strain of Rhizobium rhizogenes (formerly Agrobacterium rhizogenes), Agrobacterium rhizogenes(kan R) Ar A4 Ri(agropine type). Agrobacterium rhizogenes is a soil-borne gram-negative bacterium that can infect most dicotyledons, a few monocotyledons and some gymnosperms. Ar.A4 Chemically Competent Agrobacterium are optimized for the highest transformation efficiencies and are useful for transgenic operations of corn, tobacco, carrot and other plants. Ar.A4 Agrobacterium rhizogenes strain contains agrobacterium-type Ri plasmid and displays kanamycin resistance.

Clamp Set Chemically Resistant Rods and Clamps

MIX1267 EACH
EUR 314.4

MSU440 Chemically Competent Agrobacterium- 18x50µl

1077-36 50μL
EUR 755
Description: Intact Genomics (ig®) MSU440 Chemically Competent Agrobacterium cells are made from a specific strain of Rhizobium rhizogenes (formerly Agrobacterium rhizogenes), Agrobacterium rhizogenes(str R) MSU440 Ri (agropine type). Agrobacterium rhizogenes is a soil-borne gram-negative bacterium that can infect most dicotyledons, a few monocotyledons and some gymnosperms. MSU440 Chemically Competent Agrobacterium are optimized for the highest transformation efficiencies and are useful for transgenic operations of corn, tobacco, wormwood, tea tree and other plants. MSU440 Agrobacterium rhizogenes strain contains agrobacterium-type Ri plasmid and displays streptomycin resistance.

GV3101 Chemically Competent Agrobacterium - 18x50µl

1082-18 50μL
EUR 755
Description: GV3101 strain (A. tumefaciens) has a C58 chromosomal background with rifampicin resistance and the Ti plasmid pMP90 (pTiC58DT-DNA) with gentamicin resistance. The GV3101 Ti plasmid has the T-DNA region sequences deleted and transformation with a binary vector containing the missing T-region results in a functional T-DNA binary system that allows for transfer of genetic material into a host plant’s genome. Therefore, this system is often used for Agrobacterium-mediated transformation of several dicots such as Arabidopsis thaliana, tobacco, potato, and monocots like corn.Product Includes:• GV3101 Chemically cells• pCAMBIA control DNA• Recovery medium

EHA105 Chemically Competent Agrobacterium - 18x50µl

1084-18 50μL
EUR 755
Description: Intact Genomics EHA105 Chemically Competent Agrobacterium cells are optimized for the highest transformation efficiencies which is ideal for applications requiring high transformation efficiencies, such as with cDNA or gDNA library construction. The EHA105 strain is useful for transgenic operations of rice, tobacco and other plants. EHA105 contains a rifampicin resistance gene (rif). EHA105 strain also contains an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA) without self-transport function, which contains the vir gene.Product Includes:• EHA105 Chemically Competent cells• pCAMBIA control DNA• Recovery medium

LBA4404 Chemically Competent Agrobacterium - 6x50µl

1085-06 50μL
EUR 355
Description: Intact Genomics LBA4404 Chemically Competent Agrobacterium cells are optimized for the highest transformation efficiencies which is ideal for applications requiring high transformation efficiencies, such as with cDNA or gDNA library construction. The LBA4404 strain is useful for transgenic operations of tomatoes, tobacco and other plants. LBA4404 contains a rifampicin resistance gene (rif). LBA4404 strain also contains a octoprine-type Ti plasmid pAL4404 without self-transport function, which contains the vir gene.Product Includes:• LBA4404 Chemically Competent cells• pCAMBIA control DNA• Recovery medium

Ar. A4 Chemically Competent Agrobacterium- 18x50µl

1072-18 50μL
EUR 755
Description: Intact Genomics (ig®) Ar.A4 Chemically Competent Agrobacterium cells are made from a specific strain of Rhizobium rhizogenes (formerly Agrobacterium rhizogenes), Agrobacterium rhizogenes(kan R) Ar A4 Ri(agropine type). Agrobacterium rhizogenes is a soil-borne gram-negative bacterium that can infect most dicotyledons, a few monocotyledons and some gymnosperms. Ar.A4 Chemically Competent Agrobacterium are optimized for the highest transformation efficiencies and are useful for transgenic operations of corn, tobacco, carrot and other plants. Ar.A4 Agrobacterium rhizogenes strain contains agrobacterium-type Ri plasmid and displays kanamycin resistance.

LBA4404 Chemically Competent Agrobacterium - 18x50µl

1085-18 50μL
EUR 755
Description: Intact Genomics LBA4404 Chemically Competent Agrobacterium cells are optimized for the highest transformation efficiencies which is ideal for applications requiring high transformation efficiencies, such as with cDNA or gDNA library construction. The LBA4404 strain is useful for transgenic operations of tomatoes, tobacco and other plants. LBA4404 contains a rifampicin resistance gene (rif). LBA4404 strain also contains a octoprine-type Ti plasmid pAL4404 without self-transport function, which contains the vir gene.Product Includes:• LBA4404 Chemically Competent cells• pCAMBIA control DNA• Recovery medium

ATCC15834 Chemically Competent Agrobacterium- 6x50µl

1075-12 50μL
EUR 355
Description: Intact Genomics (ig®) ATCC15834 Chemically Competent Agrobacterium cells are made from a specific strain of Rhizobium rhizogenes (formerly Agrobacterium rhizogenes), Agrobacterium rhizogenes pRi15834 (agropine type). Agrobacterium rhizogenes is a soil-borne gram-negative bacterium that can infect most dicotyledons, a few monocotyledons and some gymnosperms. ATCC15834 Chemically Competent Agrobacterium are optimized for the highest transformation efficiencies and are useful for transgenic operations of legumes, tobacco, variety of grasses and other plants. ATCC15834 Agrobacterium rhizogenes strain contains pRi15834 agrobacterium-type Ri plasmid and displays resistance to Rifampicin.

ATCC15834 Chemically Competent Agrobacterium- 18x50µl

1075-36 50μL
EUR 755
Description: Intact Genomics (ig®) ATCC15834 Chemically Competent Agrobacterium cells are made from a specific strain of Rhizobium rhizogenes (formerly Agrobacterium rhizogenes), Agrobacterium rhizogenes pRi15834 (agropine type). Agrobacterium rhizogenes is a soil-borne gram-negative bacterium that can infect most dicotyledons, a few monocotyledons and some gymnosperms. ATCC15834 Chemically Competent Agrobacterium are optimized for the highest transformation efficiencies and are useful for transgenic operations of legumes, tobacco, variety of grasses and other plants. ATCC15834 Agrobacterium rhizogenes strain contains pRi15834 agrobacterium-type Ri plasmid and displays resistance to Rifampicin.

BL21(DE3) pLysS Chemically Competent Cells - 12x50µl

1056-12 50μL
EUR 140
Description: Intact Genomics (ig®) chemically competent BL21(DE3)pLysS chemically competent cells are suitable for transformation and routine protein expression. The pLysS plasmid produces T7 lysozyme that reduces base level expression of the gene of interest, subsequently allowing for tighter control of expression and is therefore suitable for expression of toxic genes. BL21(DE3)pLysS chemically competent cells also carry the chloramphenicol resistance gene.

BL21(DE3) pLysS Chemically Competent Cells - 12x200µl

1056-48 200μL
EUR 275
Description: Intact Genomics (ig®) chemically competent BL21(DE3)pLysS chemically competent cells are suitable for transformation and routine protein expression. The pLysS plasmid produces T7 lysozyme that reduces base level expression of the gene of interest, subsequently allowing for tighter control of expression and is therefore suitable for expression of toxic genes. BL21(DE3)pLysS chemically competent cells also carry the chloramphenicol resistance gene.

DirectPlate™ 10B Chemically Competent Cells 12x50µl

1015-12 50μL
EUR 129
Description: Intact Genomics (ig®) DirectPlate™ Competent cells offer simple, fast, and robust results for your DNA transformation needs. DirectPlate™ 10B chemically competent E. coli cells are a perfect choice for researchers looking to simplify their transformation workflow by eliminating heat shock, lengthy incubations, and time-consuming outgrowth procedures. Simply mix and directly plate! DirectPlate™ 10B chemically competent E. coli cells provide higher transformation efficiency than any competitor‘s similar product and are suitable for high-efficiency transformation in a wide variety of applications such as cloning and sub-cloning.Product Includes:DirectPlate™ 10B Chemically Competent CellspUC19 control DNA

DirectPlate™ 10B Chemically Competent Cells 36x50µl

1015-36 50μL
EUR 315
Description: Intact Genomics (ig®) DirectPlate™ Competent cells offer simple, fast, and robust results for your DNA transformation needs. DirectPlate™ 10B chemically competent E. coli cells are a perfect choice for researchers looking to simplify their transformation workflow by eliminating heat shock, lengthy incubations, and time-consuming outgrowth procedures. Simply mix and directly plate! DirectPlate™ 10B chemically competent E. coli cells provide higher transformation efficiency than any competitor‘s similar product and are suitable for high-efficiency transformation in a wide variety of applications such as cloning and sub-cloning.Product Includes:DirectPlate™ 10B Chemically Competent CellspUC19 control DNA

Human ES and iPS Complete Cell Medium (Chemically defined)

TBS8064-100ML 100mL
EUR 169

*Chemically-Derived ATA-Phe-Phe-Arg-CMK Purified

ICMFFRCK100UG each
EUR 374
Description: *Chemically-Derived ATA-Phe-Phe-Arg-CMK Purified

*Chemically-Derived ATA-Phe-Phe-Arg-CMK Purified

ICMFFRCK1MG each
EUR 3145
Description: *Chemically-Derived ATA-Phe-Phe-Arg-CMK Purified

*Chemically-Derived ATA-Phe-Pro-Arg-CMK Purified

ICMFPRCK100UG each
EUR 374
Description: *Chemically-Derived ATA-Phe-Pro-Arg-CMK Purified

*Chemically-Derived ATA-Phe-Pro-Arg-CMK Purified

ICMFPRCK1MG each
EUR 3145
Description: *Chemically-Derived ATA-Phe-Pro-Arg-CMK Purified

DirectPlate™ BL21(DE3) Chemically Competent Cells 12x50µl

1019-12 50μL
EUR 145
Description: Intact Genomics (ig®) DirectPlate™ Competent cells offer simple, fast, and robust results for your DNA transformation needs. DirectPlate™ BL21(DE3) chemically competent E. coli cells are a perfect choice for researchers looking to simplify their transformation workflow by eliminating heat shock, lengthy incubations, and time-consuming outgrowth procedures. Simply mix and directly plate! DirectPlate™ BL21(DE3) chemically competent E. coli cells provide higher transformation efficiency than any competitor‘s similar product and are suitable for high-efficiency transformation in a wide variety of applications such as cloning, protein expression and sub-cloning.Product Includes:DirectPlate™ BL21(DE3) Chemically Competent CellspUC19 control DNA

DirectPlate™ BL21(DE3) Chemically Competent Cells 36x50µl

1019-36 50μL
EUR 355
Description: Intact Genomics (ig®) DirectPlate™ Competent cells offer simple, fast, and robust results for your DNA transformation needs. DirectPlate™ BL21(DE3) chemically competent E. coli cells are a perfect choice for researchers looking to simplify their transformation workflow by eliminating heat shock, lengthy incubations, and time-consuming outgrowth procedures. Simply mix and directly plate! DirectPlate™ BL21(DE3) chemically competent E. coli cells provide higher transformation efficiency than any competitor‘s similar product and are suitable for high-efficiency transformation in a wide variety of applications such as cloning, protein expression and sub-cloning.Product Includes:DirectPlate™ BL21(DE3) Chemically Competent CellspUC19 control DNA

DirectPlate XL™Chemically Competent Agrobacterium- 6x50µl

1094-06 50μL
EUR 295
Description: Intact Genomics (ig®) DirectPlate-XL™ DH10B Competent cells offer simple, fast and robust results for your large DNA transformation needs – directly clone large DNA up to >250kb! DirectPlate-XL™ DH10B chemically competent E. coli cells are a perfect choice for researchers looking to simplify their large DNA transformation workflow by eliminating heat shock, lengthy incubations, and time-consuming outgrowth procedures. Simply mix and directly plate! DirectPlate-XL™ DH10B chemically competent E. coli cells provide higher transformation efficiency than any competitor ‘s similar product and are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. Using proprietary technology, these cells can be used for transformation of extremely large DNA (i.e. >10kb up to >250kb).

DirectPlate XL™Chemically Competent Agrobacterium- 36x50µl

1094-36 50μL
EUR 675
Description: Intact Genomics (ig®) DirectPlate-XL™ DH10B Competent cells offer simple, fast and robust results for your large DNA transformation needs – directly clone large DNA up to >250kb! DirectPlate-XL™ DH10B chemically competent E. coli cells are a perfect choice for researchers looking to simplify their large DNA transformation workflow by eliminating heat shock, lengthy incubations, and time-consuming outgrowth procedures. Simply mix and directly plate! DirectPlate-XL™ DH10B chemically competent E. coli cells provide higher transformation efficiency than any competitor ‘s similar product and are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. Using proprietary technology, these cells can be used for transformation of extremely large DNA (i.e. >10kb up to >250kb).

DH5-Alpha Chemically Competent Cells (ig 5-Alpha) - 12x50µl

1031-12 12/PK
EUR 204

DH5-Alpha Chemically Competent Cells (ig 5-Alpha)- 24x50µl

1031-24 24/PK
EUR 290.4

DH5-Alpha Chemically Competent Cells (ig 5-Alpha)- 6x200µl

1034-24 6/PK
EUR 235.2

DH5-Alpha Chemically Competent Cells (ig 5-Alpha)- 6x100µl

1032-12 6/PK
EUR 184.8

DH5-Alpha Chemically Competent Cells (ig 5-Alpha)- 12x100µl

1032-24 12/PK
EUR 278.4

DH5-Alpha Chemically Competent Cells (ig 5-Alpha)- 24x100µl

1032-48 24/PK
EUR 434.4

DH5-Alpha Chemically Competent Cells (ig 5-Alpha)- 12x200µl

1034-48 12/PK
EUR 384

DirectPlate™ DH5-Alpha Chemically Competent Cells 12x50µl

1013-12 50μL
EUR 129
Description: Intact Genomics (ig®) DirectPlate™ DH5-Alpha Competent cells offer simple, fast, and robust results for your DNA transformation needs. DirectPlate™ DH5-Alpha chemically competent E. coli cells are a perfect choice for researchers looking to simplify their transformation workflow by eliminating heat shock, lengthy incubations, and time-consuming outgrowth procedures. Simply mix and directly plate! DirectPlate™ DH5-Alpha chemically competent E. coli cells provide higher transformation efficiency than any competitor‘s similar product and are suitable for high-efficiency transformation in a wide variety of applications such as cloning and sub-cloning.Product Includes:DirectPlate™ DH5-alpha competent cellspUC19 control DNA

DirectPlate™ DH5-Alpha Chemically Competent Cells 36x50µl

1013-36 50μL
EUR 315
Description: Intact Genomics (ig®) DirectPlate™ Competent cells offer simple, fast, and robust results for your DNA transformation needs. DirectPlate™ DH5-Alpha chemically competent E. coli cells are a perfect choice for researchers looking to simplify their transformation workflow by eliminating heat shock, lengthy incubations, and time-consuming outgrowth procedures. Simply mix and directly plate! DirectPlate™ DH5-Alpha chemically competent E. coli cells provide higher transformation efficiency than any competitor‘s similar product and are suitable for high-efficiency transformation in a wide variety of applications such as cloning and sub-cloning.Product Includes:DirectPlate™ DH5-alpha competent cellspUC19 control DNA

DH10B Chemically Competent Cells (ig10B) - 1x96 well plate - 20µl/well

1018-96 1/EA
EUR 621.6

*Chemically-Derived Ac-TVASSSTA Octapeptide Purified Lyophilized

ICMACOLY10MG each
EUR 886
Description: *Chemically-Derived Ac-TVASSSTA Octapeptide Purified Lyophilized

*Chemically-Derived Ac-TVASSSTA Octapeptide Purified Lyophilized

ICMACOLY5MG each
EUR 517
Description: *Chemically-Derived Ac-TVASSSTA Octapeptide Purified Lyophilized

*Chemically-Derived Fluorescein-Phe-Pro-Arg-CMK Purified

ICMINFPR100UG each
EUR 325
Description: *Chemically-Derived Fluorescein-Phe-Pro-Arg-CMK Purified

*Chemically-Derived Fluorescein-Phe-Pro-Arg-CMK Purified

ICMINFPR1MG each
EUR 2564
Description: *Chemically-Derived Fluorescein-Phe-Pro-Arg-CMK Purified

DirectPlate™ TG1 Phage Display Chemically Competent Cells 12x50µl

1024-12 50μL
EUR 180
Description: Intact Genomics (ig®) DirectPlate™ Competent cells offer simple, fast, and robust results for your DNA transformation needs. DirectPlate™ TG1 Phage Display chemically competent E. coli cells are a perfect choice for researchers looking to simplify their transformation workflow by eliminating heat shock, lengthy incubations, and time-consuming outgrowth procedures. Simply mix and directly plate! DirectPlate™ TG1 Phage Display chemically competent E. coli cells provide higher transformation efficiency than any competitor‘s similar product and are suitable for high-efficiency transformation in a wide variety of applications such as cloning, phage display libraries and sub-cloning.Product Includes:DirectPlate™ TG1 Phage Display Chemically Competent CellspUC19 control DNA

DirectPlate™ TG1 Phage Display Chemically Competent Cells 36x50µl

1024-36 50μL
EUR 355
Description: Intact Genomics (ig®) DirectPlate™ Competent cells offer simple, fast, and robust results for your DNA transformation needs. DirectPlate™ TG1 Phage Display chemically competent E. coli cells are a perfect choice for researchers looking to simplify their transformation workflow by eliminating heat shock, lengthy incubations, and time-consuming outgrowth procedures. Simply mix and directly plate! DirectPlate™ TG1 Phage Display chemically competent E. coli cells provide higher transformation efficiency than any competitor‘s similar product and are suitable for high-efficiency transformation in a wide variety of applications such as cloning, phage display libraries and sub-cloning.Product Includes:DirectPlate™ TG1 Phage Display Chemically Competent CellspUC19 control DNA

Clamp set for H3770 Chemically resistant/inert teflon, includes rod and clamp

H3770-CSTF 1 each
EUR 236.8

DH5-Alpha Chemically Competent Cells (ig 5-Alpha)- 1x96 well plate - 20µl/well

1038-96 1/EA
EUR 621.6

Corning Chemically Resistant Sealing Mat for 96 Well Expanded Volume Microplate Nonsterile

3346 PK50
EUR 510

EC MEDIUM, MODIFIED

E05-108-10kg 10 kg
EUR 1029.6

EC MEDIUM, MODIFIED

E05-108-2Kg 2 Kg
EUR 271.2

EC MEDIUM, MODIFIED

E05-108-500g 500 g
EUR 117.6

Letheen Agar Modified

MED1656 500G
EUR 110.4

KNUDSON MODIFIED PLUS

K425 10L
EUR 173.28

LETHEEN BROTH, MODIFIED

L12-109M-10kg 10 kg
EUR 1708.8

LETHEEN BROTH, MODIFIED

L12-109M-2Kg 2 Kg
EUR 418.8

LETHEEN BROTH, MODIFIED

L12-109M-500g 500 g
EUR 157.2

Letheen Broth Modified

MED1658 500G
EUR 93.6

Wrights Stain (Modified)

RRSP153-D 500ml
EUR 9.25

Wrights Stain (Modified)

RRSP153-E 1L
EUR 12.49

Wrights Stain (Modified)

RRSP153-F 2.5L
EUR 19.31

Jenners Stain Modified

RRSP76-D 500ml
EUR 7.54

Jenners Stain Modified

RRSP76-E 1L
EUR 10.8

Jenners Stain Modified

RRSP76-F 2.5L
EUR 19.47

TERRIFIC BROTH, MODIFIED

T20-114M-10kg 10 kg
EUR 736.8

TERRIFIC BROTH, MODIFIED

T20-114M-2Kg 2 Kg
EUR 207.6

TERRIFIC BROTH, MODIFIED

T20-114M-500g 500 g
EUR 99.6

Lillies Fixative (Modified)

RRSP661-E 1L
EUR 10.07

Lillies Fixative (Modified)

RRSP661-F 2.5L
EUR 17.31

Ischemia Modified Albumin

AP80059 1mg
EUR 2640

Gill’s modified EA

09783-1000 1000ml
EUR 100
Description: 64-17-5

Gill’s modified EA

09783-3.75 3,75L
EUR 320
Description: 64-17-5

Gill’s modified EA

09783-500 500ml
EUR 61
Description: 64-17-5

McCoy's 5A Media (Modified)

CM032-050 500ml
EUR 105.6

McCoy's 5A Media (Modified)

CM032-300 6x500ml
EUR 198

McCoy's 5A Media (Modified)

CM032-310 10x500ml
EUR 262.8

Wykazano, że metodologie wibracyjne mają duży potencjał w zakresie systematycznego gromadzenia danych o ekosystemach, a uzyskaną zmienność filogenetyczną można dobrze wyjaśnić składem biochemicznym pyłku. Spośród siedmiu testowanych metod, najlepsze zróżnicowanie taksonomiczne pyłku uzyskano przy pomocy pomiarów w podczerwieni na próbkach zbiorczych, a także pomiarów obszaru korpusu ziarna pyłku metodą mikrospektroskopii Ramana. Pomiary mikrospektroskopii ramanowskiej wskazują, że obszar pomiarowy, jak również głębia ostrości, mogą mieć decydujący wpływ na otrzymane dane.