Thraustochytrids zostały odkryte po raz pierwszy w 1934 roku, a od lat 60-tych XX wieku są coraz częściej badane pod kątem ich korzystnych i szkodliwych skutków. Przegląd ten ma na celu zapewnienie lepszego zrozumienia tych protistów, ze szczególnym naciskiem na ich taksonomię, ekologię i zastosowania biotechnologiczne. Przez lata taksonomia thraustochytrid uległa poprawie wraz z rozwojem nowoczesnych technik molekularnych i nowych markerów biochemicznych, co doprowadziło do izolacji i opisu nowych szczepów. W niniejszej pracy dokonano przeglądu historii taksonomicznej thraustochytrids, zapewniając jednocześnie aktualną klasyfikację tych organizmów.
Opisuje również różne biomarkery, które można wziąć pod uwagę w celu wsparcia taksonomicznej charakterystyki thraustochytrida, wraz z przeglądem tradycyjnych i nowoczesnych technik ich izolacji i identyfikacji molekularnej. Oryginalność tego przeglądu polega na połączeniu taksonomii i ekologii thraustochytridów oraz ich biotechnologicznych zastosowań jako producentów kwasu dokozaheksaenowego (DHA), karotenoidów, egzopolisacharydów i innych interesujących związków. Przeprowadzono również eksperymenty, aby sprawdzić wykrywanie metali toksycznych. Aktywności przeciwutleniające obliczono na podstawie wartości EC50 z ekstraktów z grzybów.
Artykuł zawiera podsumowanie tych aspektów, podkreślając jednocześnie niektóre z najważniejszych ostatnich badań w tej dziedzinie, które obejmują różnorodność metabolizmu wielonienasyconych kwasów tłuszczowych w thraustochytridach, niektóre nowe strategie produkcji biomasy i odzyskiwania związków będących przedmiotem zainteresowania. Ponadto przedstawiono szczegółowy przegląd bezpośrednich i prądowych zastosowań związków pochodzących z thraustochytridu w przemyśle spożywczym, paliwowym, kosmetycznym, farmaceutycznym i akwakulturze, a także niektórych dostępnych produktów handlowych. Przegląd ten ma być źródłem informacji i odniesień na temat thraustochytrids zarówno dla ekspertów, jak i dla nowych w tej dziedzinie.
Ramaria Fr. i Clavaria L. to dwa główne rodzaje grzybów koralowych odpowiednio z rodzin Gomphaceae i Clavariaceae. Oprócz odgrywania ważnej roli w ekologii lasu, niektóre z nich mają wysoki potencjał nutraceutyczny i bioaktywny. W północno-zachodnich Himalajach istnieje ukryta różnorodność grzybów koralowych. Obecne badania opisują szczegółowe profilowanie biochemiczne oraz działanie przeciwutleniające i przeciwbakteryjne dwunastu gatunków grzybów koralowych. Biochemiczne profilowanie składników odżywczych i nutraceutyków przeprowadzono standardowymi technikami oraz przy użyciu HPLC, UPLC i GC.
Porównanie dwóch powszechnych technik przygotowania próbek do analizy lipidów i kwasów tłuszczowych w trzech różnych morfotypach koralowców ujawnia ilościowe i jakościowe różnice.
Lipidy biorą udział w wielu procesach biochemicznych i fizjologicznych w koralowcach. Dlatego zmiany w składzie lipidów odzwierciedlają zmiany w ekologii, odżywianiu i zdrowiu koralowców. W związku z tym dokładna ekstrakcja lipidów, oznaczenie ilościowe i identyfikacja mają kluczowe znaczenie dla uzyskania wszechstronnego wglądu w stan koralowca. Jednak na całym świecie istnieją rozbieżności w metodologii przygotowywania próbek i obecnie nie wiadomo, czy te techniki dają analogiczne wyniki. W tym badaniu porównano dwie najpopularniejsze techniki przygotowania próbek do analizy lipidów koralowców: (1) izolacja tkanki przez rozpylanie powietrza i (2) miażdżenie korala w całości.
Próbki pochodzące z każdej techniki przygotowania były następnie analizowane w celu oznaczenia ilościowego lipidów i ich klas składowych oraz kwasów tłuszczowych w czterech powszechnych gatunkach koralowców skleraktynowych reprezentujących trzy różne morfotypy (Acropora millepora, Montipora crassotuberculata, Porites cylindrica i Pocillopora damicornis). Wyniki ujawniły znaczne ilości materiału organicznego, w tym lipidów, zatrzymanych w szkieletach wszystkich gatunków po rozpyleniu powietrznym, powodując wyraźne niedoszacowanie całkowitego stężenia lipidów przy użyciu tej metody.
Ponadto klasa lipidów i skład kwasów tłuszczowych między odsłoniętym szkieletem a opryskaną tkanką były zasadniczo różne. W szczególności większość całkowitych stężeń triacyloglicerolu i całkowitych kwasów tłuszczowych została zachowana w szkielecie (odpowiednio 55-69% i 56-64%). W związku z tym wyizolowana, rozpylona tkanka nie może służyć jako wiarygodny wskaźnik do ilościowego oznaczania lub identyfikacji lipidów w koralowym holobioncie. Dlatego też metoda kruszenia in toto jest zalecana do przygotowania próbek koralowców przed analizą lipidów w celu uchwycenia profilu lipidowego całego holobiontu, co pozwala na dokładne zdiagnozowanie stanu koralowców.
Identyfikacja barwników powłoki u ślimaków morskich Clanculus pharaonius i C. margaritarius (Trochoidea; Gastropoda).
Kolor i wzór to kluczowe cechy, które odgrywają ważną rolę w kamuflażu, ostrzeżeniach i atrakcyjności. Idealnie, aby zacząć rozumieć ewolucję i ekologię koloru w przyrodzie, ważne jest, aby zidentyfikować i, jeśli to możliwe, w pełni scharakteryzować pigmenty przy użyciu metod biochemicznych. Gromada mięczaków obejmuje jedne z najpiękniejszych przykładów pigmentacji biologicznej, z żywymi kolorami muszli morskich, które są szczególnie cenione przez kolekcjonerów i naukowców. Biochemiczne badania koloru muszli mięczaków były dość powszechne w ostatnim stuleciu, ale niewiele z tych badań zostało potwierdzonych nowoczesnymi metodami i bardzo niewiele pigmentów skorup zostało w pełni scharakteryzowanych.
Tutaj wykorzystujemy nowoczesne chemiczne i multimodalne techniki spektroskopowe do identyfikacji dwóch pigmentów porfirynowych i eumelaniny w skorupach ślimaków morskich Clanculus pharaonius i C margaritarius. Te same porfiryny zidentyfikowano również w zabarwionej tkance stóp obu gatunków. Używamy wysokosprawnej chromatografii cieczowej (HPLC), aby ostatecznie wykazać, że te porfiryny to uroporfiryna I i uroporfiryna III. Dowody z analiz mikroskopii konfokalnej pokazują, że rozmieszczenie pigmentów porfirynowych odpowiada uderzającej różowo-czerwonej muszli C. pharaonius, a także różowo-czerwonym kropkom i liniom na wczesnych okółkach C. margaritarius oraz żółto-brązowemu kolorowi późniejszych okółków.
GeneGlide? DNA Transfection Reagent |
M1080-300 |
Biovision |
each |
EUR 322.8 |
GeneGlide? DNA Transfection Reagent |
M1080-500 |
Biovision |
each |
EUR 433.2 |
Susfectin™ Transfection Reagent |
G4000 |
ABM |
1.0 ml |
EUR 195 |
RNAifectin™ Transfection Reagent |
E36G073 |
EnoGene |
1.0 ml (100-500 transfections) |
EUR 228.57 |
Lentifectin™ Transfection Reagent |
E36G074 |
EnoGene |
1.0 ml |
EUR 214.29 |
DNAfectin™ 2100 Transfection Reagent |
E36G2100 |
EnoGene |
1.0 mg/1.0 ml |
EUR 200 |
EnoGeneFec™ 2000 Transfection Reagent |
EGF2000-1000 |
EnoGene |
1000μl |
EUR 220 |
EnoGeneFec™ 2000 Transfection Reagent |
EGF2000-1500 |
EnoGene |
1500μl |
EUR 330 |
EnoGeneFec™ 2000 Transfection Reagent |
EGF2000-500 |
EnoGene |
500μl |
EUR 130 |
EnoGeneFec™ 2100 Transfection Reagent |
EGF2100-1000 |
EnoGene |
1000μl |
EUR 240 |
EnoGeneFec™ 2100 Transfection Reagent |
EGF2100-1500 |
EnoGene |
1500μl |
EUR 350 |
EnoGeneFec™ 2100 Transfection Reagent |
EGF2100-500 |
EnoGene |
500μl |
EUR 150 |
EnoGeneFec™ 2200 Transfection Reagent |
EGF2200-1000 |
EnoGene |
1000μl |
EUR 260 |
EnoGeneFec™ 2200 Transfection Reagent |
EGF2200-1500 |
EnoGene |
1500μl |
EUR 370 |
EnoGeneFec™ 2200 Transfection Reagent |
EGF2200-500 |
EnoGene |
500μl |
EUR 170 |
EnoGeneFec™ 2300 Transfection Reagent |
EGF2300-1000 |
EnoGene |
1000μl |
EUR 270 |
EnoGeneFec™ 2300 Transfection Reagent |
EGF2300-1500 |
EnoGene |
1500μl |
EUR 380 |
EnoGeneFec™ 2300 Transfection Reagent |
EGF2300-500 |
EnoGene |
500μl |
EUR 180 |
DNAfectin™ Plus Transfection Reagent |
E36G2500 |
EnoGene |
1.0 ml |
EUR 271.43 |
DNAfectin™ Plus Transfection Reagent |
G2500 |
ABM |
1.0 ml |
EUR 195 |
EL Transfection Reagent |
20-abx098880 |
Abbexa |
|
|
|
LP4K Transfection Reagent |
LP4K |
GenTarget |
1.0 ml / vial |
EUR 364.8 |
Description: Lipid based transfection reagent for large plasmid and multiple plasmid transfection in both adhesive and suspenstion cell types. |
Convoy? Transfection Reagent |
1110-1ml |
ACTGene |
each |
EUR 409.2 |
HighGene transfection reagent |
RM09014 |
Abclonal |
1000μl |
EUR 324 |
NanoFect Transfection Reagent |
NF100 |
ALSTEM |
1 ml |
EUR 189.63 |
Transfection Reagent (1 mL) |
P901 |
101Bio |
- |
Ask for price |
Transfection Reagent (0.1 mL) |
P901S |
101Bio |
- |
Ask for price |
ExFect2000 Transfection Reagent |
T202-01 |
Vazyme |
0.5 ml |
EUR 272.4 |
ExFect2000 Transfection Reagent |
T202-02 |
Vazyme |
1 ml |
EUR 379.2 |
ExFect2000 Transfection Reagent |
T202-03 |
Vazyme |
5 ml |
EUR 1262.4 |
PureFection Transfection Reagent |
LV750A-1 |
SBI |
1 ml |
EUR 303 |
Transfectamineâ„¢ 5000 Transfection Reagent |
60020-05mL |
AAT Bioquest |
0.5 mL |
EUR 222 |
Description: Transfectamine™ 5000 Transfection Reagent is a powerful and versatile transfection reagent for the introduction of nucleic acids into eukaryotic cells, or more specifically, into animal cells. |
Transfectamineâ„¢ 5000 Transfection Reagent |
60021-1mL |
AAT Bioquest |
1 mL |
EUR 334 |
Description: Transfectamine™ 5000 Transfection Reagent is a powerful and versatile transfection reagent for the introduction of nucleic acids into eukaryotic cells, or more specifically, into animal cells. |
Transfectamineâ„¢ 5000 Transfection Reagent |
60022-5mL |
AAT Bioquest |
5 mL |
EUR 846 |
Description: Transfectamine™ 5000 Transfection Reagent is a powerful and versatile transfection reagent for the introduction of nucleic acids into eukaryotic cells, or more specifically, into animal cells. |
Transfectamineâ„¢ mRNA Transfection Reagent |
60030-500ul |
AAT Bioquest |
500 ul |
EUR 203 |
|
Description: Transfectamineâ„¢ mRNA Transfection Reagent is a powerful and versatile transfection reagent designed to introduce a higher amount of mRNA into eukaryotic cells, or more specifically, into animal cells. |
Transfectamineâ„¢ mRNA Transfection Reagent |
60031-5ml |
AAT Bioquest |
5 ml |
EUR 989 |
|
Description: Transfectamineâ„¢ mRNA Transfection Reagent is a powerful and versatile transfection reagent designed to introduce a higher amount of mRNA into eukaryotic cells, or more specifically, into animal cells. |
iMFectin DNA Transfection Reagent |
I7100-100 |
GenDepot |
100ul |
EUR 176.4 |
iMFectin DNA Transfection Reagent |
I7100-101 |
GenDepot |
1ml |
EUR 530.4 |
iMFectin DNA Transfection Reagent |
I7100-105 |
GenDepot |
5x1ml |
EUR 2247.6 |
iMFectin DNA Transfection Reagent |
I7100-120 |
GenDepot |
20ml |
EUR 8144.4 |
Lentifectin (TM) Transfection Reagent |
DNAF003 |
Bio Basic |
1ml |
EUR 334.57 |
|
Sapphire Insect Transfection Reagent |
ABP-BVD-10003 |
Allele Biotech |
75ul, 25 transfections |
Ask for price |
|
PureFection™ Transfection reagent |
LV750A-5 |
SBI |
5 ml |
EUR 1287 |
iMFectin 2000 DNA Transfection Reagent |
I7300-101 |
GenDepot |
1ml |
Ask for price |
iMFectin 2000 DNA Transfection Reagent |
I7300-105 |
GenDepot |
5x1ml |
Ask for price |
iMFectin Poly DNA Transfection Reagent |
I7200-100 |
GenDepot |
100ul |
EUR 176.4 |
iMFectin Poly DNA Transfection Reagent |
I7200-101 |
GenDepot |
1ml |
EUR 530.4 |
iMFectin Poly DNA Transfection Reagent |
I7200-105 |
GenDepot |
5x1ml |
EUR 2247.6 |
iMFectin Poly DNA Transfection Reagent |
I7200-120 |
GenDepot |
20ml |
EUR 8144.4 |
QuickShuttle-Basic Transfection Reagent |
E28X0110041 |
EnoGene |
0.8ml |
EUR 266.67 |
QuickShuttle-Enhanced Transfection Reagent |
E28X0110042 |
EnoGene |
0.8ml |
EUR 266.67 |
QuickShuttle-Superfast Transfection Reagent |
E28X0110043 |
EnoGene |
0.8ml |
EUR 266.67 |
RNAfection, RNA transfection reagent 50 ul |
MR750A-1 |
SBI |
50 ul |
EUR 157 |
QuickShuttle-293(293 cell Transfection Reagent) |
E28X0110044 |
EnoGene |
0.8ml |
EUR 266.67 |
QuickShuttle-Hela(Hela cell Transfection Reagent) |
E28X0110045 |
EnoGene |
0.8ml |
EUR 266.67 |
T-Pro Nonliposomal Transfection Reagent I (NTR I) |
JT97-N001M |
T-Pro Biotechnology |
1.0ml/vial |
EUR 224.4 |
T-Pro Nonliposomal Transfection Reagent II (NTR II) |
JT97-N002M |
T-Pro Biotechnology |
1.0ml/vial |
EUR 244.8 |
T-Pro Nonliposomal Transfection Reagent III (NTR III) |
JT97-N006M |
T-Pro Biotechnology |
1.0ml/vial |
EUR 266.4 |
QuickShuttle-BHK-21(BHK-21 cell Transfection Reagent) |
E28X0110046 |
EnoGene |
0.8ml |
EUR 266.67 |
GeneGlide? RNAi Delivery Control |
M1082-10 |
Biovision |
each |
EUR 284.4 |
GeneGlide? RNAi Delivery Control |
M1082-100 |
Biovision |
each |
EUR 1002 |
GeneGlide? RNAi Delivery Control |
M1082-50 |
Biovision |
each |
EUR 594 |
pPACK-SPIKE Combo Kit, includes Cat# CVD19-500A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
CVD19-520A-KIT |
SBI |
1 kit |
EUR 1141 |
pPACK-SPIKE D614G Combo Kit, includes Cat# CVD19-530A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
CVD19-550A-KIT |
SBI |
1 kit |
EUR 1141 |
pPACK-SPIKE N501Y Combo Kit, includes Cat# CVD19-560A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
CVD19-575A-KIT |
SBI |
1 kit |
EUR 1141 |
pPACK-SPIKE Alpha Combo Kit, includes Cat# CVD19-590A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
CVD19-599A-KIT |
SBI |
1 kit |
EUR 1141 |
pPACK-SPIKE Gamma Combo Kit, includes Cat# CVD19-630A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
CVD19-639A-KIT |
SBI |
1 kit |
EUR 1141 |
pPACK-SPIKE Beta Combo Kit, includes Cat# CVD19-640A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
CVD19-649A-KIT |
SBI |
1 kit |
EUR 1141 |
pPACK-SPIKE Delta Combo Kit, includes Cat# CVD19-650A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
CVD19-659A-KIT |
SBI |
1 kit |
EUR 1141 |
pPACK-SPIKE Omicron Combo Kit, includes Cat# CVD19-660A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
CVD19-669A-KIT |
SBI |
1 kit |
EUR 1162 |
pPACK-SPIKE B.1.429 Combo Kit, includes Cat# CVD19-610A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
CVD19-619A-KIT |
SBI |
1 kit |
EUR 1141 |
pPACK-SPIKE B.1.525 Combo Kit, includes Cat# CVD19-620A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
CVD19-629A-KIT |
SBI |
1 kit |
EUR 1141 |
Exo-Fect siRNA/miRNA Transfection Kit |
EXFT200A-1 |
SBI |
20 reactions |
EUR 412 |
pPACK-SPIKE B.1.351 RBD Mutations Combo Kit, includes Cat# CVD19-580A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
CVD19-589A-KIT |
SBI |
1 kit |
EUR 1141 |
pPACK-SPIKE B.1.351 S1 Mutations Combo Kit, includes Cat# CVD19-600A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
CVD19-609A-KIT |
SBI |
1 kit |
EUR 1141 |
Transfection Collection™ : PDE4D Transient Pack |
79286 |
BPS Bioscience |
500 rxns. |
EUR 845 |
Description: The PDE4D Transient Pack is designed to provide the tools necessary for transiently transfecting and for screening inhibitors of PDE4D7 in cultured HEK293 cells._x000D_The assay is based on transfecting cells with the CRE luciferase reporter. CRE reporter contains the firefly luciferase gene under the control of cAMP response element (CRE). Elevation of intracellular cAMP activates CRE binding protein (CREB) to bind CRE and induce the expression of luciferase. Forskolin is commonly used to raise the intracellular level of cAMP in cell physiology studies. When cells transiently transfected with CRE reporter are activated by forskolin, the intracellular level of cAMP is upregulated, which induces the expression of CRE luciferase reporter._x000D_However, when cells are co-transfected with PDE4D7 expression vector and CRE reporter, the level of forskolin-induced cAMP is reduced, resulting in lower expression level of luciferase. When cells are treated with PDE4D inhibitor to inhibit PDE4D7 activity, cAMP level is restored, resulting in higher luciferase activity. The PDE4D Transient Pack contains transfection-ready CRE luciferase reporter. This reporter contains transfection-ready vectors for PDE4D7 expression and a CRE vector containing firefly luciferase as a cAMP pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control._x000D_It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells. The key to the PDE4D Cell-Based Activity Reporter Detection Valuepack is the CRE luciferase reporter vector, which is a cAMP/PKA Cell Signaling Pathway-responsive reporter. This reporter contains the firefly luciferase gene under the control of multimerized cAMP response elements (CRE) located upstream of a minimal promoter. The vector is premixed with constitutively-expressing Renilla (sea pansy) luciferase vector that serves as an internal control for transfection efficiency. The pack also includes the PDE4D7 expression vector, and the adenylate cyclase activator, forskolin. Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 and HeLa cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep._x000D_Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D_*Note: the kit may be used with other cell lines than HEK293 or HeLa, but an alternate cell culture medium may be required for optimal cell growth |
µ-Transfection Kit VI |
K010-0.1 |
BIONTEX |
each |
EUR 628 |
FBS, Transfection Optimized |
F0640-050 |
GenDepot |
500ml |
EUR 902.4 |
Transfection Collection™ : AP1 Transient Pack (JNK Signaling Pathway) |
79266 |
BPS Bioscience |
500 rxns. |
EUR 715 |
Description: The AP1 Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of the JNK signaling pathway and the transcriptional activity of AP1 in cultured HEK293 cells. The kit contains transfection-ready vectors containing firefly luciferase as a JNK pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the AP1 Transient Pack is the AP1 luciferase reporter vector. This reporter contains the firefly luciferase gene under the control of multimerized AP1 responsive elements located upstream of a minimal promoter. The AP1 reporter is premixed with a constitutively-expressing Renilla luciferase vector that serves as an internal control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively- expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains the firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical for determining pathway-specific effects and the background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 and HeLa cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required. _x000D_*Note: the kit may be used with other cell lines than HEK293 or HeLa, but an alternate cell culture medium may be required for optimal cell growth |
Transfection Collection™ : Myc Transient Pack (Myc Signaling Pathway) |
79284 |
BPS Bioscience |
500 rxns. |
EUR 750 |
Description: The Myc Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of the Myc signaling pathway in cultured HCT116 cells. The Myc Transient Pack contains transfection-ready vectors containing firefly luciferase as a Myc pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HCT116 cells._x000D_.The key to the Myc Transient Pack is the expression vectors for c-Myc and Myc luciferase reporter vector. Inside the cells, c-Myc will bind to Max, translocate to the nucleus, and induce expression of the Myc luciferase reporter vector. This reporter contains the firefly luciferase gene under the control of multimerized Myc responsive elements located upstream of a minimal promoter. The Myc reporter is premixed with constitutively-expressing Renilla (sea pansy) luciferase vector, which serves as an internal positive control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. This negative control is critical for determining pathway-specific effects and background luciferase activity. An "empty" expression vector without the c-Myc gene is also provided as an additional negative control._x000D_Additionally, the pack includes cell culture medium that has been optimized for use with HCT116 cells*. HCT116 is a human colon cancer cell line with a mutated β -catenin which leads to the accumulation of β-catenin and constitutive activation of the Myc signaling pathway. This medium includes 10% fetal bovine serum, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D_*Note: the kit may be used with other cell lines than HCT116, but an alternate cell culture medium may be required for optimal cell growth. For HEK293 cells, we recommend using BPS Medium 1 (BPS Bioscience, #79259). |
293T Transfection Kit (1 mL) |
P902 |
101Bio |
- |
Ask for price |
293T Transfection Kit (0.2 mL) |
P902S |
101Bio |
- |
Ask for price |
293T Transfection Kit (1 mL) |
P903 |
101Bio |
- |
Ask for price |
293T Transfection Kit (0.2 mL) |
P903S |
101Bio |
- |
Ask for price |
µ-Transfection Kit VI FluoR |
K020-0.1 |
BIONTEX |
each |
EUR 720 |
Transfection Collection™ : SRE Transient Pack (MAPK/ERK Signaling Pathway) |
79271 |
BPS Bioscience |
500 rxns. |
EUR 750 |
Description: The SRE Reporter Kit Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of the ERK signaling pathway and the transcriptional activity of SRE in cultured HEK293 cells. The kit contains transfection-ready vectors containing firefly luciferase as a MAPK/ERK pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the SRE Transient Pack is the SRE luciferase reporter vector, which is an ERK pathway-responsive reporter. This reporter contains the firefly luciferase gene under the control of multimerized SRE responsive elements located upstream of a minimal promoter. The SRE reporter is premixed with a constitutively-expressing Renilla luciferase vector that serves as an internal control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively- expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains the firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical for determining pathway-specific effects and the background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D_* Other mammalian cell lines may also be used, but an alternate cell culture medium may be required for optimal cell growth. |
Transfection Collection™ : SBE Transient Pack (TGFβ/SMAD Signaling Pathway) |
79272 |
BPS Bioscience |
500 rxns. |
EUR 750 |
Description: The SBE Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of TGFβ/SMAD signaling pathway in the cultured cells. The kit contains transfection-ready vectors containing firefly luciferase as a TGFβ/SMAD pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the SBE Transient Pack is the SBE luciferase reporter vector, which is a TGFβ pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimerized SBE responsive element located upstream of a minimal promoter. The SBE reporter is premixed with constitutively expressing Renilla luciferase vector that serves as internal control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively expressing Renilla luciferase vector as negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway-specific effects and background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required. _x000D_*Note: the kit may be used with other cell lines than HEK293, but an alternate cell culture medium may be required for optimal cell growth. |
Transfection Collection™ : Notch1/CSL Transient Pack (Notch Signaling Pathway) |
79285 |
BPS Bioscience |
500 rxns. |
EUR 715 |
Description: The Notch1/CSL Transient Packis designed to provide the tools necessary for transiently transfecting and monitoring the activity of the Notch signaling pathway in HEK293 cultured cells. The kit contains transfection-ready vectors containing firefly luciferase as a Notch pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the Notch1/CSL Transient Pack is the expression vector for NOTCH1 that has a deletion of the entire extracellular domain (Notch1DE). Inside the cells, the NOTCH1 DE can be cleaved by γ-secretase and active NOTCH1 NICD is released into the nucleus. The kit also contains CSL (CBF1/RBP-Jk) luciferase reporter vector, which is a Notch pathway-responsive reporter. This reporter contains the firefly luciferase gene under the control of multimerized CSL responsive elements upstream of a minimal promoter. The CSL (CBF1/RBP-Jk) reporter is premixed with constitutively expressing Renilla (sea pansy) luciferase vector, which serves as an internal positive control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. The negative control is critical for determining pathway specific effects and background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required. _x000D_*Note: the kit may be used with other cell lines than HEK293, but an alternate cell culture medium may be required for optimal cell growth, |
Exo-Fect Exosome Transfection Kit |
EXFT10A-1 |
SBI |
10 reactions |
EUR 238 |
Exo-Fect Exosome Transfection Kit |
EXFT20A-1 |
SBI |
20 reactions |
EUR 429 |
Transfection Collection™ : NF-κB Transient Pack ( NF-κB Signaling Pathway) |
79268 |
BPS Bioscience |
500 rxns. |
EUR 780 |
Description: The NF-κB Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of the NF-κB signaling pathway in cultured HEK293 cells. The kit contains transfection-ready vectors containing firefly luciferase as a NF-κB pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the NF-κB Transient Pack is the NF-κB luciferase reporter vector. This reporter contains a firefly luciferase gene under the control of multimerized NF-κB responsive element located upstream of a minimal promoter. The NF-κB reporter is premixed with constitutively-expressing Renilla luciferase vector, which serves as an internal control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway specific effects and background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D_*Note: the kit may be used with other cell lines than HEK293, but an alternate cell culture medium may be required for optimal cell growth. |
The Transfection Collection™ : ISRE Transient Pack JAK/STAT Signaling Pathway |
79264 |
BPS Bioscience |
500 rxns. |
EUR 715 |
Description: The ISRE Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of Type I interferon-induced JAK/STAT signaling pathway in cultured HEK293 cells. The pack contains transfection-ready vectors containing firefly luciferase as a JAK/STAT pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the ISRE Transient Pack is the ISRE luciferase reporter vector, which is a JAK/STAT pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimerized ISRE responsive element located upstream of a minimal promoter. The ISRE reporter is premixed with constitutively-expressing Renilla luciferase vector, which serves as an internal control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway specific effects and background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D_*Note: the kit may be used with other cell lines than HEK293, but an alternate cell culture medium may be required for optimal cell growth. |
K2® Transfection System 1 x 200 µl |
T060-0.2 |
BIONTEX |
200 ul |
Ask for price |
K2® Transfection System 1 x 750 µl |
T060-0.75 |
BIONTEX |
750 ul |
EUR 231 |
K2® Transfection System 1 x 1.5 ml |
T060-1.0 |
BIONTEX |
1.5 ml |
EUR 393 |
K2® Transfection System 2 x 1.5 ml |
T060-2.0 |
BIONTEX |
1.5ml |
EUR 739 |
K2® Transfection System 5 x 1.5 ml |
T060-5.0 |
BIONTEX |
1.5 ml |
EUR 1733 |
K4® Transfection System 1 x 200 µl |
T080-0.2 |
BIONTEX |
200 ul |
Ask for price |
K4® Transfection System 1 x 1 ml |
T080-1.0 |
BIONTEX |
1 ml |
EUR 260 |
K4® Transfection System 2 x 1 ml |
T080-2.0 |
BIONTEX |
1 ml |
EUR 491 |
K4® Transfection System 5 x 1 ml |
T080-5.0 |
BIONTEX |
1 m; |
EUR 1155 |
Calcium Phosphate Cell Transfection Kit |
K1028-100 |
ApexBio |
100T |
EUR 30 |
Description: Transfection Kit, Calcium phosphate transfection method |
Calcium Phosphate Cell Transfection Kit |
K1028-200 |
ApexBio |
200T |
EUR 50 |
Description: Transfection Kit, Calcium phosphate transfection method |
The Transfection Collection™ : GAL4 Transient Pack Glucocorticoid Receptor Pathway |
79265 |
BPS Bioscience |
500 rxns. |
EUR 715 |
Description: The GAL4 Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of the glucocorticoid signaling pathway in cultured HEK293 cells. The kit contains transfection-ready vectors containing firefly luciferase as a glucocorticoid pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the GAL4 Transient Pack is the expression vector for the glucocorticoid receptor ligand binding domain that is fused to the DNA binding domain (DBD) of GAL4 (GAL4 DBD-GR). This fusion construct activates firefly luciferase expression under the control of a multimerized GAL4 upstream activation sequence (UAS). This allows for specific detection of glucocorticoid-induced activation of the glucocorticoid receptor without the need for individual transcriptional targets and with low cross-reactivity for other nuclear receptor pathways. The GAL4/UAS reporter is premixed with constitutively expressing Renilla (sea pansy) luciferase vector, which serves as an internal positive control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. The negative control is critical for determining pathway-specific effects and background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D_*Note: the kit may be used with other cell lines than HEK293, but an alternate cell culture medium may be required for optimal cell growth. |
Calcium Phasphate Transfection Kit (Small) |
TBS8201 |
Tribioscience |
Kit |
EUR 118 |
Calcium Phasphate Transfection Kit (Large) |
TBS8201-2 |
Tribioscience |
Kit |
EUR 328 |
Transfection Collection™ : CRE/CREB Transient Pack (cAMP/PKA Cell Signaling Pathway) |
79267 |
BPS Bioscience |
500 rxns. |
EUR 715 |
Description: The CRE/CREB Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of the cAMP/PKA signaling pathway in cultured cells. The kit contains transfection-ready vectors containing firefly luciferase as a cAMP/PKA pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the CRE/CREB Transient Pack is the CRE/CREB luciferase reporter vector, which is a cAMP/PKA Cell Signaling Pathway-responsive reporter. This reporter contains the firefly luciferase gene under the control of multimerized cAMP response elements (CRE) located upstream of a minimal promoter. Elevation of the intracellular cAMP level activates cAMP response element binding protein (CREB) to bind CRE and induces the expression of luciferase._x000D_The CRE reporter is premixed with constitutively-expressing Renilla (sea pansy) luciferase vector that serves as an internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with the constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains the firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. The negative control is critical to determining pathway-specific effects and background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D_* Other mammalian cell lines may also be used, but an alternate cell culture medium may be required for optimal cell growth. |
Transfection Collection™ : TCF/LEF Transient Pack Wnt / β-catenin Signaling Pathway |
79273 |
BPS Bioscience |
500 rxns. |
EUR 750 |
Description: The TCF/LEF Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of Wnt / Beta-catenin signaling pathway in the cultured HEK293 cells. The TCF/LEF Transient Pack contains transfection-ready vectors containing firefly luciferase as a Wnt pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells._x000D_The key to the TCF/LEF Transient Pack is the TCF/LEF luciferase reporter vector, which is a Wnt pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimerized TCF/LEF responsive element located upstream of a minimal promoter. The TCF/LEF reporter is premixed with constitutively expressing Renilla luciferase vector that serves as internal control for transfection efficiency._x000D_The pack also includes a non-inducible firefly luciferase vector premixed with constitutively expressing Renilla luciferase vector as negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway specific effects and background luciferase activity._x000D_Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D_*Note: the kit may be used with other cell lines than HEK293, but an alternate cell culture medium may be required for optimal cell growth. |
Transfection-ready Cas9 SmartNuclease AAVS1 gRNA |
CAS520A-1 |
SBI |
10 ug |
EUR 281 |
Sapphire Baculovirus DNA and Transfection Kit |
ABP-BVD-10002 |
Allele Biotech |
1 kit |
Ask for price |
|
Dodatkowe wyniki HPLC sugerują, że za czarne plamy prawdopodobnie odpowiada eumelanina. Aby je rozróżnić, określamy dwa różnie zabarwione pigmenty porfirynowe jako trochopuniceus (różowo-czerwony) i trochoxouthos (żółto-brązowy). Trochopuniceus i trochoxouthos nie zostały znalezione w skorupie trzeciego gatunku z tej samej nadrodziny Calliostoma zizyphinum, pomimo jej powierzchownie podobnego zabarwienia, co sugeruje, że ten gatunek ma różne pigmenty muszli. Odkrycia te mają ważne implikacje dla badania koloru i wzoru w szczególności mięczaków, ale bardziej ogólnie w innych taksonach, ponieważ to badanie pokazuje, że homologii widocznego koloru nie można założyć bez identyfikacji pigmentów.