May 3, 2024

High-yield preparation of isolated rat liver parenchymal cells: a biochemical and fine structural study.

A new technique employing continuous recirculating perfusion of the rat liver in situ, shaking of the liver in buffer in vitro, and filtration of the tissue through nylon mesh, results in the conversion of about 50% of the liver into intact, isolated parenchymal cells. The perfusion media consist of: (a) calcium-free Hanks’ solution containing 0.05% collagenase and 0.10% hyaluronidase, and (b) magnesium and calcium-free Hanks’ solution containing 2 mM ethylenediaminetetraacetate. Biochemical and morphologic studies indicate that the isolated cells are viable.

Rat Adipose Mesenchymal Stem Cells

CP-R198 5×105 cells/vial
EUR 580
Description: Rat

Mouse Adipose Mesenchymal Stem Cells

CP-M138 5×105 cells/vial
EUR 610
Description: Mouse

Rat Endosteal Mesenchymal Stem Cells

CP-R161 5×105 cells/vial
EUR 580
Description: Rat

Rabbit Adipose Mesenchymal Stem Cells

CP-Rb028 5×105 cells/vial
EUR 640
Description: Rabbit

Mouse Endosteal Mesenchymal Stem Cells

CP-M171 5×105 cells/vial
EUR 610
Description: Mouse

Mesenchymal Stem Cells - Adipose Derived

SC00AD1 500,000 Cells
EUR 1086

Rabbit Endosteal Mesenchymal Stem Cells

CP-Rb014 5×105 cells/vial
EUR 610
Description: Rabbit

Mesenchymal Stem Cells - Placental Derived

SC00P1 500,000 cells
EUR 1086

Mesenchymal Stem Cells-Adipose Derived

MBS556462-INQUIRE INQUIRE Ask for price

Mesenchymal Stem Cells-Placental Derived

MBS556468-INQUIRE INQUIRE Ask for price

Rat Bone Marrow Mesenchymal Stem Cells

CP-R131 5×105 cells/vial
EUR 580
Description: Rat

Human Mesenchymal Stem Cells Wharton's Jelly (HMSC-WJ)

CSC-C4087X One Frozen vial Ask for price

Mouse Bone Marrow Mesenchymal Stem Cells

ABC-TC3916 1 vial Ask for price
Description: Mouse bone marrow mesenchymal stem cells, 6-week ICR mouse

Mouse Bone Marrow Mesenchymal Stem Cells

CP-M131 5×105 cells/vial
EUR 580
Description: Mouse

Human Bone Marrow Mesenchymal Stem Cells

HUXMA-01001 1*10^6/vial
EUR 1030

Human Mesenchymal Stem cells Bone Marrow

ALHE31 1ml frozen Vial
EUR 620

Rabbit Bone Marrow Mesenchymal Stem Cells

ABC-TC4058 1 vial Ask for price
Description: Rabbit bone marrow mesenchymal stem cells, 5-week rabbit

Canine Bone Marrow Mesenchymal Stem Cells

CAXMX-01001 1*10^6/vial
EUR 999

Rabbit Bone Marrow Mesenchymal Stem Cells

CP-Rb007 5×105 cells/vial
EUR 610
Description: Rabbit

Rabbit Bone Marrow Mesenchymal Stem Cells

RBXMX-01001 1*10^6/vial
EUR 649

Mesenchymal Stem Cells, Mouse, Basal Medium

MBS653076-500mL 500mL
EUR 420

Mesenchymal Stem Cells, Mouse, Basal Medium

MBS653076-5x500mL 5x500mL
EUR 1740

Mesenchymal Stem Cells - Bone Marrow Derived

SC00BM1 500,000 cells
EUR 1086

Human Umbilical Cord Mesenchymal Stem Cells

HUXUC-01001 1*10^6/vial
EUR 1159

Human iPSC-derived Mesenchymal Stem Cells

CSC-00205L One Frozen vial Ask for price

Mesenchymal Stem Cells, Mouse, Freezing Medium

MBS653422-50mL 50mL
EUR 435

Mesenchymal Stem Cells, Mouse, Freezing Medium

MBS653422-5x50mL 5x50mL
EUR 1740

Mesenchymal Stem Cells-Bone Marrow Derived

MBS556467-INQUIRE INQUIRE Ask for price

CD-1 Mouse Lung Mesenchymal Stem Cells

ABC-TC3293 1 vial Ask for price
Description: Mouse Lung Mesenchymal Stem Cells are derived from the lung tissues of pathogen-free laboratory adult mice. Mouse Lung Mesenchymal Stem Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 7-15 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Mouse Lung Mesenchymal StemCells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded 3-8 passages by the ratio of 1:3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Mouse Lung Mesenchymal Stem Cells are tested for expression of markers using antibodies, CD44, Sca-1 and CD29 positive by flow cytometry.Standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.

Mesenchymal Stem Cells, MSCGM, T-150 FSK

LOPT-2501T150 each Ask for price

SD Rat Bone Marrow Mesenchymal Stem Cells

RASMX-01001 1*10^6/vial
EUR 479

Human Adipose-Derived Mesenchymal Stem Cells

CR1004-500 1 vial
EUR 725
Description: Cryopreserved vial of human adipose-derived mesenchymal stem cells (500,000 cells)

Human Adipose-derived Mesenchymal Stem Cells

HUXMD-01001 1*10^6/vial
EUR 1079

C57BL/6 Mouse Lung Mesenchymal Stem Cells

ABC-TC3213 1 vial Ask for price
Description: Mouse Lung Mesenchymal Stem Cells are derived from the lung tissues of pathogen-free laboratory adult mice. Mouse Lung Mesenchymal Stem Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 7-15 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen. Mouse Lung Mesenchymal StemCells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded 3-8 passages by the ratio of 1:3 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Mouse Lung Mesenchymal Stem Cells are tested for expression of markers using antibodies, CD44, Sca-1 and CD29 positive by flow cytometry.Standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.

Canine Adipose-derived Mesenchymal Stem Cells

CAXMD-01001 1*10^6/vial
EUR 1200

Rabbit Adipose-derived Mesenchymal Stem Cells

RBXMD-01001 1*10^6/vial
EUR 799

F344 Rat Bone Marrow Mesenchymal Stem Cells

RAFMX-01001 1*10^6/vial
EUR 479

CD1 Mouse Bone Marrow Mesenchymal Stem Cells

CSC-00800L One Frozen vial Ask for price

Wistar Rat Bone Marrow Mesenchymal Stem Cells

RAWMX-01001 1*10^6/vial
EUR 479

Human Fetal Bone Marrow Mesenchymal Stem Cells

HUXMF-01001 1*10^6/vial
EUR 1200

Rat Primary Bone Marrow Mesenchymal Stem Cells

T4835 5x10^5 cells / 1.0 ml
EUR 925

Immortalized Human Wharton’s Jelly derived Mesenchymal Stem Cells

CSC-I2031Z One Frozen vial Ask for price

SD Rat Adipose-derived Mesenchymal Stem Cells

RASMD-01001 1*10^6/vial
EUR 639

Human Mesenchymal Stem Cells Expressing Oct3/4

SC00A6 500000
EUR 1420.8

Human Primary Bone Marrow Mesenchymal Stem Cells

T4019 5x10^5 cells / 1.0 ml Ask for price

Human Mesenchymal Stem Cells - Bone Marrow Derived

MSC001 500,000+ Cells
EUR 1545.6

Mouse Primary Bone Marrow Mesenchymal Stem Cells

T4535 10^6 cells / 1.0 ml Ask for price

Human Mesenchymal Stem Cells-hepatic (HMSC-he)

CSC-7755W One Frozen vial Ask for price

Human Mesenchymal Stem Cells-adipose (HMSC-ad)

CSC-7757W One Frozen vial Ask for price

Human Umbilical Cord Blood Mesenchymal Stem Cells

HUXUB-01001 1*10^6/vial
EUR 1159

Diabetic Mouse Bone Marrow Mesenchymal Stem Cells

CSC-00801L One Frozen vial Ask for price

Rat Adipose Mesenchymal Stem Cells Complete Medium

CM-R198-100mL 100 mL
EUR 98
Description: Complete Growth Medium

Rat Adipose Mesenchymal Stem Cells Complete Medium

CM-R198-125mL4 125 mL×4
EUR 388
Description: Complete Growth Medium

Human Bone Marrow-Derived Mesenchymal Stem Cells

CR1005-500 1 vial
EUR 725
Description: Cryopreserved vial of human bone marrow-derived mesenchymal stem cells (500,000 cells)

Rat Adipose Mesenchymal Stem Cells Complete Medium

CM-R198 100mL
EUR 98
Description: Primary cells complete growth medium

Human Bone Marrow-Derived Mesenchymal Stem Cells

cAP-0050 1Frozen Vial
EUR 594

Rat Adipose Mesenchymal Stem Cells Complete Medium

MBS2568976-100mL 100mL
EUR 155

Rat Adipose Mesenchymal Stem Cells Complete Medium

MBS2568976-4x125mL 4x125mL
EUR 360

Human Mesenchymal Stem Cells-Bone Marrow Derived

MBS556170-INQUIRE INQUIRE Ask for price

Rat Bone Marrow Mesenchymal Stem Cells With GFP

RBXMX-01101 1*10^6/vial
EUR 1089

Rat Bone Marrow Mesenchymal Stem Cells With RFP

RBXMX-01201 1*10^6/vial
EUR 1089

Wistar Rat Adipose-derived Mesenchymal Stem Cells

RAWMD-01001 1*10^6/vial
EUR 639

BALB/c Mouse Bone Marrow Mesenchymal Stem Cells

ABC-TC3059 1 vial Ask for price
Description: Mouse Bone Marrow Mesenchymal Stem Cells are derived from the tibias and femurs of pathogen-free laboratory adult mice. Mouse Bone Marrow Mesenchymal Stem Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 7-15 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 2x10^6 cells per ml and is delivered frozen.Mouse Bone Marrow Mesenchymal Stem Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Each batch of Mouse Bone Marrow Mesenchymal Stem Cells are tested for expression of markers using antibodies, CD44, Sca-1 and CD29 by flow cytometry.Standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.

Balb/c Mouse Bone Marrow Mesenchymal Stem Cells

MUCMX-01001 1*10^6/vial
EUR 639

BALB - c Mouse Bone Marrow Mesenchymal Stem Cells

CSC-C4348X One Frozen vial Ask for price

Mouse Adipose Mesenchymal Stem Cells Complete Medium

CM-M138-100mL 100 mL
EUR 98
Description: Complete Growth Medium

Mouse Adipose Mesenchymal Stem Cells Complete Medium

CM-M138-125mL4 125 mL×4
EUR 388
Description: Complete Growth Medium

Rat Endosteal Mesenchymal Stem Cells Complete Medium

CM-R161-100mL 100 mL
EUR 98
Description: Complete Growth Medium

Rat Endosteal Mesenchymal Stem Cells Complete Medium

CM-R161-125mL4 125 mL×4
EUR 388
Description: Complete Growth Medium

Mouse Adipose Mesenchymal Stem Cells Complete Medium

CM-M138 100mL
EUR 98
Description: Primary cells complete growth medium

Rat Endosteal Mesenchymal Stem Cells Complete Medium

CM-R161 100mL
EUR 98
Description: Primary cells complete growth medium

Mouse Adipose Mesenchymal Stem Cells Complete Medium

MBS2568935-100mL 100mL
EUR 155

Mouse Adipose Mesenchymal Stem Cells Complete Medium

MBS2568935-4x125mL 4x125mL
EUR 360

Rat Endosteal Mesenchymal Stem Cells Complete Medium

MBS2568970-100mL 100mL
EUR 155

Rat Endosteal Mesenchymal Stem Cells Complete Medium

MBS2568970-4x125mL 4x125mL
EUR 360

C57BL/6 Mouse Bone Marrow Mesenchymal Stem Cells

ABC-TC3172 1 vial Ask for price
Description: Mouse Bone Marrow Mesenchymal Stem Cells are derived from the tibias and femurs of pathogen-free laboratory adult mice. Mouse Bone Marrow Mesenchymal Stem Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium for 7-15 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 2x10^6 cells per ml and is delivered frozen.Mouse Bone Marrow Mesenchymal Stem Cells are negative for bacteria, yeast, fungi, and mycoplasma.Cells can be expanded on a multiwell culture platetandard biochemical procedures performed with cell ready for experiments under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Each batch of Mouse Bone Marrow Mesenchymal Stem Cells are tested for expression of markers using antibodies, CD44, Sca-1 and CD29 by flow cytometry.Standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.

Human Bone Marrow Mesenchymal Stem Cells With GFP

HUXMA-01101 1*10^6/vial
EUR 1479

Human Bone Marrow Mesenchymal Stem Cells With RFP

HUXMA-01201 1*10^6/vial
EUR 1479

C57BL/6 Mouse Bone Marrow Mesenchymal Stem Cells

MUBMX-01001 1*10^6/vial
EUR 639

C57BL - 6 Mouse Bone Marrow Mesenchymal Stem Cells

CSC-C4296X One Frozen vial Ask for price

Rabbit Adipose Mesenchymal Stem Cells Complete Medium

CM-Rb028-100mL 100 mL
EUR 98
Description: Complete Growth Medium

Rabbit Adipose Mesenchymal Stem Cells Complete Medium

CM-Rb028-125mL4 125 mL×4
EUR 388
Description: Complete Growth Medium

Rabbit Adipose Mesenchymal Stem Cells Complete Medium

CM-Rb028 100mL
EUR 98
Description: Primary cells complete growth medium

Human Adipose Tissue-Derived Mesenchymal Stem Cells

cAP-0051 1Frozen Vial
EUR 594

Human Umbilical Cord-Derived Mesenchymal Stem Cells

cAP-0052 1Frozen Vial
EUR 594

Human Umbilical Cord-derived Mesenchymal Stem Cells

CSC-00813L One Frozen vial Ask for price

Human Adipose Tissue-Derived Mesenchymal Stem cells

ALHE23 1ml frozen Vial
EUR 620

Mesenchymal Stem Cells, Mouse, Growth Medium Supplement

MBS653119-35mL 35mL
EUR 465

Mesenchymal Stem Cells, Mouse, Growth Medium Supplement

MBS653119-5x35mL 5x35mL
EUR 1870

Rabbit Adipose Mesenchymal Stem Cells Complete Medium

MBS2568994-100mL 100mL
EUR 155

Rabbit Adipose Mesenchymal Stem Cells Complete Medium

MBS2568994-4x125mL 4x125mL
EUR 360

Canine Bone Marrow Mesenchymal Stem Cells With GFP

CAXMX-01101 1*10^6/vial
EUR 1229

Canine Bone Marrow Mesenchymal Stem Cells With RFP

CAXMX-01201 1*10^6/vial
EUR 1229

Human Mesenchymal Stem Cells bone marrow (HMSC-bm)

P0004011 One Frozen vial
EUR 870

Mouse Endosteal Mesenchymal Stem Cells Complete Medium

CM-M171-100mL 100 mL
EUR 98
Description: Complete Growth Medium

Mouse Endosteal Mesenchymal Stem Cells Complete Medium

CM-M171-125mL4 125 mL×4
EUR 388
Description: Complete Growth Medium

Human Amniotic Membrane Derived Mesenchymal Stem Cells

CR1006-500 1 vial
EUR 725
Description: Cryopreserved vial of human amniotic membrane-derived mesenchymal stem cells (500,000 cells)

Mouse Endosteal Mesenchymal Stem Cells Complete Medium

CM-M171 100mL
EUR 98
Description: Primary cells complete growth medium

Mesenchymal Stem Cells, Mouse, Normal, Cryopreserved (mMSC)

MBS652609-500KCells 500KCells
EUR 1145

Mesenchymal Stem Cells, Mouse, Normal, Cryopreserved (mMSC)

MBS652609-5x500KCells 5x500KCells
EUR 4930

Mouse Endosteal Mesenchymal Stem Cells Complete Medium

MBS2568938-100mL 100mL
EUR 155

Mouse Endosteal Mesenchymal Stem Cells Complete Medium

MBS2568938-4x125mL 4x125mL
EUR 360

Immortalized Dog Mesenchymal Stem Cells-HPV E6/E7

T0352 1x106 cells / 1.0 ml
EUR 3950

Rabbit Endosteal Mesenchymal Stem Cells Complete Medium

CM-Rb014-100mL 100 mL
EUR 98
Description: Complete Growth Medium

Rabbit Endosteal Mesenchymal Stem Cells Complete Medium

CM-Rb014-125mL4 125 mL×4
EUR 388
Description: Complete Growth Medium

Rabbit Endosteal Mesenchymal Stem Cells Complete Medium

CM-Rb014 100mL
EUR 98
Description: Primary cells complete growth medium

Rabbit Endosteal Mesenchymal Stem Cells Complete Medium

MBS2568986-100mL 100mL
EUR 155

Rabbit Endosteal Mesenchymal Stem Cells Complete Medium

MBS2568986-4x125mL 4x125mL
EUR 360

Human Umbilical Cord Mesenchymal Stem Cells With GFP

HUXUC-01101 1*10^6/vial
EUR 1599

Human Mesenchymal Stem Cells-bone marrow (HMSC-bm)

CSC-7756W One Frozen vial Ask for price

mouse bone marrow mesenchymal stem cells (B129 MBMMSC)

CSC-C3236 One Frozen vial Ask for price

Human Mesenchymal Stem Cells-Red Fluorescent-Labeled

SC00A3 500000
EUR 1154.4

Immortalized Human Pancreatic Mesenchymal Stem Cells - Myc

T0169 1x106 cells / 1.0 ml Ask for price

Immortalized Human Pancreatic Mesenchymal Stem Cells - Ras

T0170 1x106 cells / 1.0 ml
EUR 3950

SD Rat Bone Marrow Mesenchymal Stem Cells With GFP

RASMX-01101 1*10^6/vial
EUR 929

SD Rat Bone Marrow Mesenchymal Stem Cells With RFP

RASMX-01201 1*10^6/vial
EUR 929

Immortalized Human Pancreatic Mesenchymal Stem Cells - SV40

T0167 1x106 cells / 1.0 ml
EUR 3950

Rat Bone Marrow Mesenchymal Stem Cells Complete Medium

CM-R131-100mL 100 mL
EUR 98
Description: Complete Growth Medium

Rat Bone Marrow Mesenchymal Stem Cells Complete Medium

CM-R131-125mL4 125 mL×4
EUR 388
Description: Complete Growth Medium

Balb/c Mouse Adipose-derived Mesenchymal Stem Cells

MUCMD-01001 1*10^6/vial
EUR 649

Rat Bone Marrow Mesenchymal Stem Cells Complete Medium

CM-R131 100mL
EUR 98
Description: Primary cells complete growth medium

Rat Bone Marrow Mesenchymal Stem Cells Complete Medium

MBS2568965-100mL 100mL
EUR 155

Rat Bone Marrow Mesenchymal Stem Cells Complete Medium

MBS2568965-4x125mL 4x125mL
EUR 360

Human Mesenchymal Stem Cells-Green Fluorescent-Labeled

SC00A2 500000
EUR 1154.4

Immortalized Human Pancreatic Mesenchymal Stem Cells - hTERT

T0168 1x106 cells / 1.0 ml
EUR 3950

F344 Rat Bone Marrow Mesenchymal Stem Cells With GFP

RAFMX-01101 1*10^6/vial
EUR 929

F344 Rat Bone Marrow Mesenchymal Stem Cells With RFP

RAFMX-01201 1*10^6/vial
EUR 929

Human Adipose-derived Mesenchymal Stem Cells With GFP

HUXMD-01101 1*10^6/vial
EUR 1479

Human Adipose-derived Mesenchymal Stem Cells With RFP

HUXMD-01201 1*10^6/vial
EUR 1479

C57BL/6 Mouse Adipose-Derived Mesenchymal Stem Cells

MUBMD-01001 1*10^6/vial
EUR 649

Human Mesenchymal Stem Cells from Umbilical Cord Matrix

CSC-C1223 One Frozen vial Ask for price

Mesenchymal Stem Cells, Mouse, Growth Medium Kit, BioAssay

MBS653093-1Kit 1Kit
EUR 550

Mesenchymal Stem Cells, Mouse, Growth Medium Kit, BioAssay

MBS653093-5x1Kit 5x1Kit
EUR 2320

They respire in a medium containing calcium ions, synthesize glucose from lactate, are impermeable to inulin, do not stain with trypan blue, and retain their structural integrity. Electron microscopy of biopsies taken during and after perfusion reveals that desmosomes are quickly cleaved. Hemidesmosome-containing areas of the cell membrane invaginate and appear to pinch off and migrate centrally.

Tight and gap junctions, however, persist on the intact, isolated cells, retaining small segments of cytoplasm from formerly apposing parenchymal cells.